About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.
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short and efficient list there canalon
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
Isn't the first thing to do is a Gram stain? And then find out if it is anaerobic, aerobic, or facultative? then look at it under a microscope for its shape? what about the media that it can grow on? put different antibiotics in the media and see if it still can grow on it?
Of course kolean, you are right this is a good way. Although I would disagree with the use of antibiotics as they are not species specific. Although the microscopic observation (gram, shape, capsule...) is usually done after the bacteria has been grown so you should know by then if it is aerobic or not. Then a group of selective media either prepared by hand or in a kit (Biomerieux API, BD crystal...) selected on the basis of the first observations.
But my method works well too. I f you have the right equipment. Might be faster too.
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
Gram's stain is certainly useful in classification but not always truthful. Some organisms, Acinetobacter in particular, can readily stain gram-positive in fresh clinical material, yet stains gram-negative after growth on plated media.
shouldn't infusion of centenarian stem cells impart longevity?
There's also API method for biochemical characteristics http://en.wikipedia.org/wiki/Analytical_Profile_Index
Every man is a star whose light can make shadows dance differently and change our view of landscape permanently***
Might be, but this takes lot of time, you need to be skillfull and often you get only the genus.
Basically, many PCR methods might be usefull and as wrote canalon, 16S sequencing is used, as it is specific and you do not need to cultivate the bacteria (so you do not risk the contaminaton or that the bacteria won't grow)
Cis or trans? That's what matters.
Give you some useful links....maybe it helps you...
http://science.jrank.org/pages/711/Bact ... teria.html
http://issuu.com/buglady/docs/17._ident ... n_bacteria
this might help
11 posts • Page 1 of 1
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