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Polynculeation

Discussion of all aspects of cellular structure, physiology and communication.

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Polynculeation

Postby softmatters » Thu Jun 04, 2009 2:28 pm

I have a culture of Mouse fibroblast cells. When I seed them on to a glass coverslip and observe them in an inverted microscope I see lots of cells with 2 or 3 nuclei and sometimes the nuclei appear to be enlarged. What is going on? Please help a lowly biophysicist.

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Postby MrMistery » Fri Jun 12, 2009 7:16 pm

well you may be observing a late telophase, when the nucleus has divided but cytokinesis has not taken place yet. or you may be observing the process of entosis - your cell with two nuclei might actually be a cell inside another cell
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