constuction of a full length gene

[shivakumar]

hi everybody
how to construct a full length gene from partial length cDNAs available from sutracted cDNA libraries. What are the techniques involved in it. And suggest me some related websites, plz.

thanks a lot in advance

[MrMistery]

well there might be a smarter way to do this, but you could just PCR them up with ends that can be cut by different restriction enzymes, so that the site on the end of one partial copy matches the site at the beginning of the next one. Then cut them, column-purify, and ligate.