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constuction of a full length gene

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constuction of a full length gene

Postby shivakumar » Tue Apr 28, 2009 5:52 am

hi everybody
how to construct a full length gene from partial length cDNAs available from sutracted cDNA libraries. What are the techniques involved in it. And suggest me some related websites, plz.

thanks a lot in advance
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Postby MrMistery » Thu Apr 30, 2009 12:04 am

well there might be a smarter way to do this, but you could just PCR them up with ends that can be cut by different restriction enzymes, so that the site on the end of one partial copy matches the site at the beginning of the next one. Then cut them, column-purify, and ligate.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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