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Single PCR tube DNA Extraction MethodModerator: BioTeam
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Single PCR tube DNA Extraction MethodI need to make a DNA extraction Method that fits in a single 150ul PCR tube and can be PCRed following the completion of the method. So, here are my ideas and method and please comment and give suggestions.
PCR Tube- Mixed inside PCR tube 10ul of 1% SDS solution 10ul of Protein K Kinase 15ul of Nucleas free water. 15 Beads from a Zippy Fecal Extraction kit, Then Vortexted at the highestl level for 30 minutes. PCR tube then Placed in a Boiling water bath for 15 miniutes then, 35ul of TAQ mastermix 1ul of fwr primer 1ul of rev primer Then PCRed. Comments or suggestions?
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