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Sequencing

Everything on bioinformatics, the science of information technology as applied to biological research.

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Sequencing

Postby Ajay » Thu Apr 16, 2009 12:42 am

A human has two sets of chromosomes and during sequencing is there a technique in which they differentiate
the two sets and individually do the sequencing ?
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Postby canalon » Thu Apr 16, 2009 2:57 am

Have you read how sequencing is done? Only one fragment at a time is sequenced, there could be 32 chromosomes, that would not impact the sequencing it self. But assembly might be slightly tricky...
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Postby Ajay » Thu Apr 16, 2009 5:25 am

sorry let me rephrase my question how exactly is the assembly done ? I am asking this because how specifically do they detect heterozygosity ?
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Postby mith » Fri Apr 17, 2009 5:03 pm

You look at the fragments and then you pick areas where they overlap, given enough copies you should be able to build a contiguous consensus based on overlaps. Theoretically you could have a bunch of frags as short as 4bps long and still be able to get a decent length of sequence.
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Postby canalon » Fri Apr 17, 2009 8:09 pm

I second mith until the last sentence. If your sequences are too short, it will be nearimpossible to get a consensus because they won't differ enough to provide enough significant overlap. Sequences the size of a PCR primer (for the exact same reason) would probably be the minimum to assemble something.
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Postby bryanwilliamz » Tue Jun 16, 2009 10:41 am

Actually the main point is that you look the fragments and check out the overlapping, and then you should be able to build a consensus, depending on overlaps.
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