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How could DNF break ETS?

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How could DNF break ETS?

Postby victor » Fri Jul 01, 2005 12:24 pm

They said that DNF (Dinitrophenol) can brak ETS and causing no ATP formed (only in the ETS, but in glycolysis and Krebs there's no problem)..how is the mechanism??
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Postby Jelanen » Fri Jul 01, 2005 6:02 pm

From http://www.ruf.rice.edu/~bioslabs/studies/mitochondria/mitopoisons.html


The compound 2,4-dinitrophenol (DNP) acts as a proton ionophore, that is, it binds protons on one side of a membrane, and being fat-soluble it drifts to the opposite side where it loses the protons. Actually, the associations/dissociations are random, but the probability of binding is greatest on the side of the membrane with greatest proton concentration, and least on the side with the lesser concentration. Thus, it is impossible to maintain a proton gradient with sufficient DNP in the system.

DNP is known to have mixed actions, that is, it produces other effects in addition to uncoupling. DNP gradually inhibits electron transport itself as it is incorporated into mitochondrial membranes. The effects appear to depend on concentration of DNP and of mitochondria, and vary from one preparation to the next.

Back in the 1930s DNP was touted as an effective diet pill. Indeed, the uncoupling of electron transport from ATP synthesis allows rapid oxidation of Krebs substrates, promoting the mobilization of carbohydrates and fats, since regulatory pathways are programmed to maintain concentrations of those substrates at set levels. Since the energy is lost as heat, biosynthesis is not promoted, and weight loss is dramatic. However, to quote Efraim Racker (A New Look at Mechanisms in Bioenergetics, Academic Press, 1976, p. 155), ..."the treatment eliminated not only the fat but also the patients,...This discouraged physicians for awhile..."

It is not a good idea to mess with cellular metabolism.


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Postby MrMistery » Fri Jul 01, 2005 8:51 pm

WOW!! Great explination Jelanen
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Postby victor » Sat Jul 02, 2005 1:33 pm

Wow... :shock: THAT'S AWESOME..!!!! :lol:
it's a great poison...more poisonous that our mod named Poison.. :lol:
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Postby MrMistery » Sat Jul 02, 2005 5:28 pm

I wouldn't bet on that :wink:
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Postby Poison » Sat Jul 02, 2005 6:41 pm

Hey!!! Did someone call my name? :) Gossip is bad, guys... :lol:
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Postby victor » Mon Jul 04, 2005 7:53 am

I'm not gossipping.....I'm just comparing... :lol:
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Postby Poison » Tue Jul 05, 2005 4:16 pm

Ok. Then I can learn your opinions about me this way!!! :lol:
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Postby MrMistery » Tue Jul 05, 2005 9:23 pm

Come on Ozge... You know we love you...
*Hope my girlfriend never joins this forum :D *
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Postby victor » Wed Jul 06, 2005 11:56 am

Why Andrew?I think it's good for her knowledge... :lol: Um, is it because of Ozge?? :lol: :P
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Postby Poison » Wed Jul 06, 2005 4:28 pm

Believe me guys, there is no point on Andrew's gf to be jealous of me. ;)

I love you all too guys. :) Or was there an irony on your post, Andrew? :lol:
*Hope my bf never joins this forum, but anyway, his english is not very good. :) OH, :? thought a second time, I don't know a single person not to understand the meaning of 'I love you'* :D
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Postby MrMistery » Wed Jul 06, 2005 5:47 pm

No irony Poison, i was serious... It's a net-love(don't ask me what that is!). Kinda like a frienship only without the other person asking if he/she can sleep over every day :D
But we should stop, this thread is seriosly going off-topic
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