why does taq add an "A"

by **theendperson** » Sat Mar 28, 2009 11:43 am

Hiya I wanted to know why taq polymerase add an A to the end of a transcript

Thanks in advance

Tom

by **plasmodesmata11** » Mon Mar 30, 2009 12:15 am

is that something to do with a poly adenyl tail?

by **theendperson** » Mon Mar 30, 2009 8:27 am

dont think so, as far as I can tell it only adds 1 A

by **canalon** » Mon Mar 30, 2009 3:43 pm

I don't know why. But it is very convenient for TA cloning. And it seems to be a unique chareacteristic of the Taq polymerase, others like Pfu do not do that. I have never seen any explanation for the why.

by **MrMistery** » Tue Mar 31, 2009 3:34 am

I really doubt it actually has a role, honestly. I think it's much more probable that it's simply a result of drift in evolution... But this is just my opinion

why does taq add an "A"

why does taq add an "A"

Re: why does taq add an "A"

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