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DNA ligases

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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DNA ligases

Postby SysBio » Fri Mar 27, 2009 3:40 am

After you "cut" DNA fragments using restriction enzymes, and then are "pasting" them together using DNA ligase...

do you need to select a specific DNA ligase that matches with the specific restriction enzyme that was used?
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Postby Sepals » Fri Mar 27, 2009 11:44 am

No. It'll anneal any broken strand. The vector is prevented from self-ligation by alkaline phosphatase.
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