Lipoprotein metabolism

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Hi. I have two questions about lipoprotein metabolism in rats and having trouble approaching them. Any help would be appreciated.

1. Rats are nocturnal feeders. How does this effect on the study of lipoprotein metabolism if the samples are collected during the normal feeding period of humans?

2. If the rats are given less chow to consume, how will this effect the VLDL metabolism of the experimental group (on liquid sucrose diet) and the control group (normal diet)?