Biology-Online • View topic - Why does DNA fluoresce under UV light?

Join for Free!
122463 members

Why does DNA fluoresce under UV light?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Why does DNA fluoresce under UV light?

Postby dae » Wed Mar 18, 2009 5:11 am

I read that it is the DNA in body fluids that make them fluoresce under UV light but I'm not sure why. If that's true, why do we need to add ethidium bromide to visualize DNA gels, wouldn't the DNA be visible by itself? Is it just a mater of intensity? I also heard that body fluids have to be dry in order to fluoresce, is that true and why would that be the case?

Posts: 27
Joined: Sun Mar 26, 2006 4:42 am

Re: Why does DNA fluoresce under UV light?

Postby CXCR4 » Wed Mar 18, 2009 7:31 pm

Wow that seems like that would be cool , do you mean like a black light fluoresce.
User avatar
Posts: 23
Joined: Tue Feb 03, 2009 12:26 am
Location: South East Coast of the United States

Postby mith » Wed Mar 18, 2009 8:35 pm

EtBr makes it fluoresce more.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
User avatar
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 2 guests