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Restriction Mapping HELP

Genetics as it applies to evolution, molecular biology, and medical aspects.

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Restriction Mapping HELP

Postby pfdunlop » Mon Feb 23, 2009 11:09 pm

I have been asked to draw a restriction map that accounts for the follwing info:-
the LINEAR dna is 1000bp
it is cut by 4 restriction enzymes E, B, P and S
the fragment sizes that are produced when these enzymes are used singly or in combination are shown int eh table below
E-227, 773
B-150,450,400
P-400,600
S-206-794
E+B-223,227,400,150
E+P-227,373,400
B+S-56,150,344,450
P+S-194,206,600

I have never been shown this before and my lecturer basically said here it is get on with it. What I want to know, is I start with the first single cutter (E) and then work out the P and S from that. but i'm not really sure after that. Do I dis-count the ones that have been cut at the same spot by more than 1, like the 400.

HELP!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
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Postby pfdunlop » Mon Feb 23, 2009 11:19 pm

Please don't reply with "do your own homework". I wish I could, I have scoured the internet for examples of the same question I am being asked to do with no avail. At the very least can someone point me in the right direction . Cheers
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Postby nena77 » Thu Feb 26, 2009 2:45 pm

the map is

150bp B 56bp S 194 bp P 150 bp B 223 bp E 227bp

to get it, I first designed 4 maps and then I combined them together. You have to start with the map of E, the map of B and the combined E+B. Then you designed the first map.
Then you take the map of P, the map of S and the one of S and P together and you combined them... and so on. You should have four maps at the end that needed to be combined in one map.

may be there is a better method, but this is the one I used.
Elena
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Postby pfdunlop » Thu Feb 26, 2009 3:37 pm

woohoo
I managed to get one of the PhDs to help me and I managed to pretty much get it myself. It wasnt easy but I did it. and i got the same answer as you. Thanks
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