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Primers for HLA typing

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Primers for HLA typing

Postby marko1985 » Sun Feb 22, 2009 7:35 pm

Hello,

for HLA typing you can use sequence specific primers from Invitrogen or any other company.
But the sequence of these primers is a "business secret" therefore you have to order the whole kit if you want them.

I do not need the official kit because my HLA typing is not for clinical or diagnostics purposes, but just for research.

Is it possible to get the sequence of these primers by sequencing or something similar? After I would have the sequence I would just order these primers by myself. I really need these primers in bulk.

Thanks for the help,
regards,
Marco
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Postby Sepals » Tue Feb 24, 2009 1:33 pm

Look up the gene you want in genebank (on the NCBI website) and order the complete opposite sequence just flanking the region you want to amplify. Alternatively you can paste the sequence into the program Vector NTI, which basically does the same thing.
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Postby marko1985 » Sun Mar 01, 2009 10:47 pm

Ok,

but is there a way to get the exact sequence of the primers in the kit?
I do not want to make new one, I just want the sequence of the ones in the kit...

thanks,
marco
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