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incubation of fungi

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Re: incubation of fungi

Postby JorgeLobo » Mon Feb 16, 2009 7:17 pm

Indoor air - dust will have osmophiles. Air from the exterior will carry Asperigillus, Penicillium and Cladosproium spore.

As biohazard suggested, the temp is prob still to high. What medium are you using?
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Postby stopherlogic » Mon Feb 16, 2009 7:24 pm

Malt extract agar
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Postby JorgeLobo » Tue Feb 17, 2009 1:51 am

Good medium for that purpose. Assume you're not that interested in osmophiles?
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Postby stopherlogic » Wed Feb 18, 2009 11:17 am

Just interested in possible pathogens really thought it was the best general purpose media. Didn't want to be too selective.
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Re: incubation of fungi

Postby JorgeLobo » Wed Feb 18, 2009 11:37 am

No worries for osmophiles if you're looking for "pathogens." Are you also looking for toxigenic fungi?
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Postby stopherlogic » Wed Feb 18, 2009 4:44 pm

Yes, anything which you would say has an effect on human health but it will be necessary to identify whatever I find.
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Postby JorgeLobo » Thu Feb 19, 2009 10:17 am

malt extract will prob be adequate. How do you plan to id - and th what extent (genus, species?)
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Postby stopherlogic » Thu Feb 19, 2009 11:28 pm

I was planning to just id using colony morphology, and use the adhesive tape method to observe microscopically, not sure whether i will need to stain as yet. Just going to categorise by genus.
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Re: incubation of fungi

Postby JorgeLobo » Fri Feb 20, 2009 11:43 am

Understand you may have limitations in what's available but colony morphology is not going to serve well, even if you're an experienced mycologist, and no way will you be able to adequately id "pathogens" even fumigatus. Scotch tape is messy and it's real hard to get decent conidiophores.

Can you set up slides culture? That would help seeing mode of spore production. Establishing higher temp incubation would help with fumigatus and a black fungus or two.

In terms of "pathogenic fungi" what do you think you'd need to id?
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Postby stopherlogic » Sat Feb 21, 2009 9:43 am

Pathogenic fungi is of particular interest which is probably why I made the mistake of incubating too high in the first place. So at 37 all I think I am seeing is a few colonies of penicillium up to now. I plan incubate the same samples at 25 on monday and hopefully I will get some more growth if everything hasn't died off. I realise pathogens are an unlikely prospect but human health was the original angle which I was taking with my project. I haven't thought about slide cultures but I will look into it. I am running low on time though.
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Re: incubation of fungi

Postby JorgeLobo » Sat Feb 21, 2009 12:12 pm

short of marneffii, what penicillium, would you have considered pathogenic?
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Postby stopherlogic » Sat Feb 21, 2009 2:18 pm

There are no others as far as I am aware and the chances of it being marneffii are unlikely. Finding pathogens is extremely unlikely unless I count opportunistic pathogens such as some aspergillus (?) which I would have to incubate at a lower temperature. I was looking for pathogens or anything affecting human health but have to identify anything I find.
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