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Fungal DNA extrtaction problem

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Fungal DNA extrtaction problem

Postby shin2 » Sat Feb 07, 2009 8:44 am

Dear all :?:
I am trying to extract DNA (size >30kb) from a filamentous fungi. no mater what technique i used the DNA size is always less then 15 kb. I will be thankful if any one can help me and send me a protocol that is capable of extracting greater then 30 kb fragment DNA

Thank you
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Postby domwood » Sun Feb 15, 2009 3:11 pm

I'm probably totally wrong here, but how much do you know about the fungal DNA. 15kb piece seems to me to be coincidentally 50% of 30kb. Is it possible the the DNA in the fungus is two separate chromosomes?
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Re: Fungal DNA extrtaction problem

Postby shin2 » Mon Feb 16, 2009 2:32 pm

I am really sorry i did not pick your point, if you could explain clearly? I have compared my extracted DNA with Limbda DNA (Hind III digested) and 1 kb DNA ladder. all the time its greater then 10 kb band and less then 24 kb of Limbda DNA.
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