Login

Join for Free!
118251 members


Crysectioning thymus

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

Crysectioning thymus

Postby BP180 » Thu Feb 05, 2009 8:33 pm

Hello all,

I would like to cryosection a mouse thymus for immunofluorescence. Does anyone know how "deep" I should start in the thymus if I want to see the medullary and thymic epithelial cells? Say my mouse thymus is about 5mm thick...can I start 1 mm in? Should my sections be along a rostal-caudal axis?

Thank you!
BP180
Garter
Garter
 
Posts: 1
Joined: Thu Feb 05, 2009 8:29 pm

Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 3 guests