Join for Free!
121591 members

Enzyme-linked immunosorbent assay(ELISA)

User contributed biology related movies.

Moderator: BioTeam

Enzyme-linked immunosorbent assay(ELISA)

Postby loyolite » Thu Jan 29, 2009 3:52 am

ELISA, Enzyme ImmunoAssay or EIA, is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality control check in various industries. In simple terms, in ELISA an unknown amount of antigen is affixed to a surface, and then a specific antibody is washed over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal. Thus in the case of fluorescence ELISA, when light of the appropriate wavelength is shone upon the sample, any antigen/antibody complexes will fluoresce so that the amount of antigen in the sample can be inferred through the magnitude of the fluorescence.

Posts: 1
Joined: Mon Sep 01, 2008 11:34 am

Re: Enzyme-linked immunosorbent assay(ELISA)

Postby leilaroozbeh » Tue May 05, 2009 10:33 am

i am interested to have a movie (animation) of ELISA that is a immunological assay
Posts: 1
Joined: Tue May 05, 2009 10:09 am

Return to Biology Movies

Who is online

Users browsing this forum: No registered users and 0 guests