primers in reading frame

[minabayardi]

HI! ¡HOLA!

I HAVE A QUESTION ABOUT THE TOPIC. IF SOME ONE CAN HELP ME, PLEASE, I NEED IT. I AM DESIGNING PCR OLIGOS, AND IN THE FUTURE WE COULD USE THEM TO SCREENING A DNA LIBRARY. IS TOTALLY IMPORTANT THAT THE PRIMERS ARE IN READING FRAME? IF THE ANSWER IS YES, PLEASE TELL ME HOW TO DO IT.
THANKS.
I AM VERY SORRY BECAUSE OF MY BAD ENGLISH, PLEASE IMAGINE THAT IS NOT BAD AT ALL. SORRY.

MINA.

[canalon]

No.

But you also want to stop posting all in capital letters. On the internet, it is similar to shouting. And that is rude.

[minabayardi]

Well, I did not know... I do not wanted be rude in any posible way. I have to apologize for do not know this rule.

Mina