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Q10, temperature coefficient

For discussing the functions of different structures of all organisms.

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Q10, temperature coefficient

Postby BioCore » Mon Dec 01, 2008 10:41 pm

Hi,

I am trying to calculate the temperature coefficient or Q10 for a cellular respiration experiment that I performed in my physiology lab using a manometer system. Everything seems to be fine in my results except for the Q10 which I keep getting (for both organisms I studied) below 1.0.

I know that Q10 is defined as:

rate of reaction (n+10oC) / rate of reaction (noC).

Now in my lab, the TA said we should use our graphs and calculate the initial slope to obtain the reaction rates, but in this way: mL O2 / g tissue / min.

I always thought rate was x/y or mL O2 consumed/min? Could this be the error that I keep getting in my Q10 or is it something else?

Thanks for your help.
BioCore
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Postby BioCore » Tue Dec 02, 2008 12:11 am

Ok it is me again, I just wanted to relay that I finally got the values to somehow equate perfectly. I think I mixed up the conversion ratios for time and volume.

I do have one more question for this lab. When I do obtain the Q10 values for both plant and animal, I get values that are really close to 1.2, which would mean that cellular respiration is limited by physical processes, or the diffusion of oxygen through the mitochondrial membrane. My question is if this is correct and what is expected. Also is there any recent research or some past research that I can read up to understand better why this is so. Thank you so much.
BioCore
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