About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.
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What is the best general purpose media for both bacteria and fungi, I have had a read around and found that nutrient agar will support the growth of most bacterial species. I also think that it may be malt agar as far as fungi is concerned.
Could someone please tell me whether I am right?
(Or a point in the right direction would be appreciated)
agar is not a nutrient. Agar is the stuff you grow your cultures on - the physical support if you wish. It is preferred precisely because there are very few bacterial species that can break it down, and no yeasts as far as I know. Malt agar will probably be ok to grow both bacteria and yeast as long as they are wild-types. That means that your bugs need to synthesize everything they need (amino acids, nucleotides etc) by themselves. It also very prone to contamination, as it has no antibiotic and anything can grow there.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
Andrew Nutrient Agar is a culture medium. Relatively rich, it is used for general purpose, just as LB could be. BHI Agar which is very rich could also be used, and will allow the growth of some more fastidious organisms. But it will not cover the needs of all cultivable bacteria, and conditions (aerobic, microaerophylic, anaerobic, temperature) are also important.
But whatever agar is used only a tiny fraction of the diversity of bacteria can be successfuly cultured.
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
Ups, sorry about that. "Nutrient agar" is kind of a weird name for a medium. But anyway, sorry for the sidetracking.
For yeast the richest medium I use is YPD(I think it stands for yeast peptone dextrose) medium. But don't really know much about it, I only use it to recover yeast after transformations, don't actually grow anything in it.
Yup, I also grew my little tiny red bacteria on nutrient agar slant. In my country, culture of bacteria usually uses Nutrient Agar (NA) or Nutrient Broth (NB). And for culture of mold, we usually use potato dextrose agar (PDA)
Some people prefer to use branded PDA, but my lecturer prefer to make a potato extract by herself and then mixed with dextrose (or table sugar) and agar powder
The result shows that self-make PDA is better than branded PDA
Q: Why are chemists great for solving problems?
A: They have all the solutions.
the point with ready-made stuff is that you don't waste a whole bunch of time doing it yourself. For example, ready-cast gels save me 3-4 hours for every gel I run, ready-made media saves me god knows how much, ready-poured plates save me half an hour, ready-cut blotting paper saves me an extra 3 minutes and the pain of cutting it wrongly etc.
8 posts • Page 1 of 1
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