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Haematocrit and Packed Cell Volumes

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Haematocrit and Packed Cell Volumes

Postby micco13 » Mon Oct 06, 2008 8:53 am

Hi there,

What is the significance of lower % PCVs or higher % PCVs in blood (mixed with saline solutions)? We did an experiment and there is a decreasing trend between PCV and NaCl ... Does the low PCV indicate that more cells has lysed (at the higher NaCl conc)? How would you tell which concentration of saline is isotonic? :?

Your help will be much appreciated
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Postby MrMistery » Fri Oct 17, 2008 2:51 am

0.9% is isotonic. And you would expect more cell lysis at lower concentrations...
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Re: Haematocrit and Packed Cell Volumes

Postby MichaelXY » Fri Oct 17, 2008 3:39 am

How would you tell which concentration of saline is isotonic?


Cell appearence should give a clue.
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