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Conservation of samples/DNA during an expedition

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Conservation of samples/DNA during an expedition

Postby Katanaji » Thu Sep 18, 2008 9:36 am

Hi!

I am preparing my first expedition. I will be going to Africa to study mycorrhizae.

To do so, I will be taking cores around trees.

I probably wont have much of a lab available, and it will be several weeks before I am back to England or the USA.

What would you advise?
I was told that I could maybe sort my roots and fungi on site and use CTAB buffer, but the method seems quite complicated and apparently requires a lot of sample material, so I am not sure if it can be applied to microscopic roots...

Any idea is welcome!

Thanks a lot :)
Katanaji
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Postby canalon » Fri Sep 19, 2008 2:04 am

You could probably resuspend your samples in tubes containing a reagent that would denature proteins like Guanidium isothiocyanate (GITC) or Guanidium Hydrochloride and freeze/refrigerate yoour samples until you go back to your lab.
Patrick

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any proof. (Ashley Montague)
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