Login

Join for Free!
118238 members


Urgent: info ELISA/EIA kit

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Urgent: info ELISA/EIA kit

Postby DoctorStein » Mon Aug 25, 2008 6:49 am

Hello,

I need ELISA/EIA kit to detect triiodothyronin (T3) level and the other one for thyroxin (T4) level of mouse and rat. It is easy to get ones for human, but so far I found difficulties to found the ones for mouse and rat. Hopefully I would get clue from you here.

Thanks in advance! :)
DoctorStein
Viper
Viper
 
Posts: 68
Joined: Fri May 30, 2008 9:27 am

Re: Urgent: info ELISA/EIA kit

Postby blcr11 » Mon Aug 25, 2008 6:28 pm

Is rat or mouse T3 or T4 iodinated at different points or something? T3 and T4 are not proteins so, unless there is something chemically unique about them, I would think an assay for the human hormones would work just as well in rodents--all other things being equal, like, for example, there's no kind of rodent interfering molecule that scarfs up the human Elisa reagent, or some wierd thing like that.
blcr11
Viper
Viper
 
Posts: 672
Joined: Fri Mar 30, 2007 4:23 am

Postby DoctorStein » Tue Aug 26, 2008 6:27 am

But from the package insert of regular insulin kit I got, it is said that the kit will result 0 % cross link with rat and mouse insulin? It seems to me that the kit doesn't work for rodents' insulin. Or it means something else? I'm sorry my English is limit.
DoctorStein
Viper
Viper
 
Posts: 68
Joined: Fri May 30, 2008 9:27 am


Postby blcr11 » Tue Aug 26, 2008 12:23 pm

Yes, but insulin is a protein and the sequences of rat, human, and mouse insulin are different and may not be (apparently aren't) recognized by the Eliza antibodies. T3 and T4 are iodinated steroid-like molecules, not peptides or protein. There shouldn't be any difference in their structures from animal to animal--unless the points at which the iodine atoms are attached differs between species. I wouldn't think so, but I don't know that for a fact.
blcr11
Viper
Viper
 
Posts: 672
Joined: Fri Mar 30, 2007 4:23 am

Postby MrMistery » Thu Aug 28, 2008 8:31 pm

they aren't(according to my course on hormones)
T3: 3,3',5-triiodo-L-thyronine
T4: 3,3',5,5'-tetraiodothyronine
In all animals they appear.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
User avatar
MrMistery
Inland Taipan
Inland Taipan
 
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 7 guests