cells surface staining

[tregis6]

to assess the % of gamma/delta T cells in flow cytometry , i use 10µl instead of 20µl/500000 cells of monoclonal antibodies as recommend by the manufacturing. Now i am aking if that will infuence my results.
thanks for helps

[Istria]

Welll, it could. Why not check it out doing both ways on a sample first?