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DNA attachment to tubes

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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DNA attachment to tubes

Postby samkh918 » Fri Jun 20, 2008 2:48 am

Hello,

I use QIAGEN Plasmid Maxi kit to extract plasmid DNA from bacterial cells. In the final steps of the procedure I have to use a 30ml tube to elute the DNA with 15ml of buffer and then add 10.5ml of isopropanol to it to precipitate the DNA. Should I use plastic Flacon tubes or Glass tubes? I want the one with less DNA attachments to the tube so that I minimize the loss of DNA? Does DNA attach more to plastic or glass?!

Thanks for your help.
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Re: DNA attachment to tubes

Postby MichaelXY » Sat Jun 21, 2008 7:35 am

Does DNA attach more to plastic or glass?!


Just a guess mind you, but I would think glass would have less bonding, based on the crystal lattice structure of Si (Si in lattice form is stable). Like I said though, just a guess.
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