Question help

[biology_06er]

Hi there,

Can someone pls help with the following questions? I've written down what I think (altho its prob wrong) but any help will be most appreciated!

what are the limitations/advantages of animal bioassays?....is it basically just stuff like accuracy, we used organ baths in our labs and many times out tension was different or stuff like wrong concnetrations via pipetting..and the advantages being things like able to determine the effects various drugs have on the tissue and able to calculate their potency?

Advantages/disadvantages of in vivo animal studies vs. cells?...in vivo studies lets us see what happens to the whole animal (knockout exps) whilst cell just shows what happens to the tissue it was extracted from

disadvantages of hetologous expression systems? (no idea bout this one)

[mith]

cost

[biology_06er]

Hi,

they are all worth 10 marks each so dnt think cost is gna get me thing ...any other ideas?

b_06er

[canalon]

No think about the cost of the assays. Raising animals vs keeping cells... But their are other reason, like the complexity of whole animal (intake, product life, etc..) that you pointed out or complex effects that cells cannot really mimick (ex: read about the botulinum toxin assay)

[biology_06er]

ahh ok, thx!...

i hv a few more that i need help with:

what are the advantages and disadvantages of autoradiography or immunohistochemistry over homogenate binding?...disadvantages of homogenate binding will include non-specific binding and also there has to be a radioactive form of the ligand being studied whilst immunohisto and autoradiography will not have these things to worry about. advantages for histo. and immuno aare that amplification can occure dur to the secondary antibody etc......any other info will be soo helpful!

the disadvantages/advantages of immunohisto and autoradiography (AG)...is it just that with immuno visualization is more easier cos it just invovles a microscope as opposed to in AG you have to use "bigger machines"...need help on this question as well

also..both these q's were 10marks each so im thinking if i wrote in my exam wat i hv above i wld get really low..so please help me!!..

cheers,
b_06er