Lowery's method V/S Bradford's method

[ss_kalve]

Hi,
plz anyone tell me for protein estimation which the best method Lowery's method or Bradford's method any why?

[blcr11]

The two assays are very similar. The main difference is the specifics of the dye used in each assay. The Bradford assay is faster, maybe a little less sensitive, and subject to more substances that interfere with the assay compared to a comparable Lowry determination. There are kits for both.

[lara]

'and subject to more substances that interfere with the assay '...sorry i didn't get that..

[MrMistery]

Bradford uses coumassie brilliant blue, which gets blue in contact with some stuff other than proteins. For example, if your mix contains detergents those will interact with the bradford reagent just like if it contained proteins. But of course most of the problems of this type are eliminated by using controls if you look for increase/decrease in protein concentration. The problems come when you are looking for an absolute result.