Silly question regarding BioEdit

[Snuggles]

I'm currently working on the rabies genome, and after the RT-PCR I sequence a region using both the forward and the reverse primers.

How do I get BioEdit to "pair up" these sequences so that I get a double stranded sequence from the forward and reverse sequences?

[mith]

we used sequencher, they had a "consensus" command.

[Snuggles]

Ok, but let's say that I only have access to BioEdit?

[MichaelXY]

You might try emailing the author of Bioedit.
[email protected]

[Snuggles]

I came right in the end, thanks anyways.

[kbowles]

In Bioedit line up the reverse sequence by editing it into the reverse complement form. It will now align with your fw sequence. Select the sequence you want, on the function tabs go into "sequence" --> "nucleic acid" --> "reverse complement"

I realize this is an old discussion but it might be helpful...

[merv]

In Bioedit line up the reverse sequence by editing it into the reverse complement form. It will now align with your fw sequence. Select the sequence you want, on the function tabs go into "sequence" --> "nucleic acid" --> "reverse complement"

I realize this is an old discussion but it might be helpful...

I think there is a similar command in Gene Jockey.
Also, if you prefer submission, you can do so : http://www.bioinformatics.org/sms/rev_comp.html