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Silly question regarding BioEdit

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Silly question regarding BioEdit

Postby Snuggles » Wed May 21, 2008 3:35 pm

I'm currently working on the rabies genome, and after the RT-PCR I sequence a region using both the forward and the reverse primers.

How do I get BioEdit to "pair up" these sequences so that I get a double stranded sequence from the forward and reverse sequences?
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Postby mith » Wed May 21, 2008 6:40 pm

we used sequencher, they had a "consensus" command.
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Postby Snuggles » Thu May 22, 2008 8:27 am

Ok, but let's say that I only have access to BioEdit?
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Postby MichaelXY » Thu May 29, 2008 1:39 am

You might try emailing the author of Bioedit.
thall@isisph.com
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Postby Snuggles » Thu May 29, 2008 7:02 am

I came right in the end, thanks anyways. :)
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Re: Silly question regarding BioEdit

Postby kbowles » Fri Apr 15, 2011 2:02 pm

In Bioedit line up the reverse sequence by editing it into the reverse complement form. It will now align with your fw sequence. Select the sequence you want, on the function tabs go into "sequence" --> "nucleic acid" --> "reverse complement"

I realize this is an old discussion but it might be helpful...
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Re: Silly question regarding BioEdit

Postby merv » Sun Oct 02, 2011 9:09 pm

kbowles wrote:In Bioedit line up the reverse sequence by editing it into the reverse complement form. It will now align with your fw sequence. Select the sequence you want, on the function tabs go into "sequence" --> "nucleic acid" --> "reverse complement"

I realize this is an old discussion but it might be helpful...


I think there is a similar command in Gene Jockey.
Also, if you prefer submission, you can do so : http://www.bioinformatics.org/sms/rev_comp.html
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