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vehicle treated apoptosis

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vehicle treated apoptosis

Postby biology_06er on Sat May 10, 2008 2:35 pm

Hi there,

Just wondering, we had Neuro2A cells treated with water and had to count the # or apoptotic cells...technically there should not be any...buuut we had like 5ish..what are sum reasons for this..just spontaneous?

cheers,
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Postby snowcapk on Sat May 10, 2008 8:35 pm

Just plain water? Maybe osmotic shock.
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Postby biology_06er on Sat May 10, 2008 10:25 pm

yep...well we had to make incubations with buffer and wash it out then add again etc with a step of adding cresyl violet..so is osmotic shock still the case?
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Postby MrMistery on Mon May 12, 2008 4:46 pm

I'm confused. Did you use distilled water or a buffer solution?
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