Login

Join for Free!
118907 members


Gene methylation

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Gene methylation

Postby B10Student » Thu May 08, 2008 5:28 am

What does methylating a sequence of DNA do to the DNA?
Last edited by B10Student on Wed Jun 11, 2008 2:53 am, edited 1 time in total.
B10Student
Garter
Garter
 
Posts: 9
Joined: Wed Dec 12, 2007 2:29 am

Postby canalon » Thu May 08, 2008 2:46 pm

sequencing is finding the the sequence/order of A[denosine], G[uanosine], C[ytosine] & T[hymidine] that makes up the DNA. Cloning it in bacterial plasmid allow faster copying because bacteria will make copying each fragment faster (think of each plasmid as a unique page that is fed in a photocopy machine, each fragment/page is fed in one photocopier very fast, OTOH if you have to make plenty of copies of the whole genome it is just like taking a heavy leather bound book and copying it without damage. Very slow). For the details you can start here:
http://en.wikipedia.org/wiki/Sequencing
or, of course, there:
http://www.biology-online.org/1/5_DNA.htm

Depending on what you are looking for (whole genome vs single gene) the use of a probe can be useful to detect which bacteria contains the plasmid that has your fragment of interest. The probe can be radioactive, but other system (biotinylation, Digoxygenine relying on immuno detection are available).

Transformation in the laboratory is carried out by Heat shock or electroporation (the latter is more efficient, but more costly in terms of equipment and cell preparation, but it is mor fun when it is failing). But many bacteria are even able to simply pick-up DNA in the environment. Not that the efficiency would be even close to useful in the lab though.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby tittu » Tue May 13, 2008 1:43 am

you use biolistics (gene gun), microinjections etc..
tittu
Garter
Garter
 
Posts: 14
Joined: Sat May 10, 2008 2:27 am



Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 1 guest