Login

Join for Free!
112251 members


interpreting a TEM

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

interpreting a TEM

Postby MrMistery » Wed May 07, 2008 8:41 pm

I know this sounds stupid(mainly because it is)
What might a TEM of a drak circle with a lot of white circles in it represent? I have no idea of the magnification, nor have I ever seen the picture. Any imput would be welcome, although I realize it is next to impossible to deduce anything from this description....
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
User avatar
MrMistery
Inland Taipan
Inland Taipan
 
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)

Postby canalon » Thu May 08, 2008 2:51 pm

Good guess about the next to impossible to deduce. I guess It would be the image of a round thing that take the stain fairly well with other structures taking it much less on it ;) A scan would be helpful.

I won't be more helpful if you do not guess what my qPCR result curve means without further description :P
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby MrMistery » Thu May 08, 2008 3:10 pm

Like i said, i have never seen the picture. If I get a copy, i'll upload it
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
User avatar
MrMistery
Inland Taipan
Inland Taipan
 
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)


Postby canalon » Thu May 08, 2008 3:19 pm

Soyou were offered the same challenge as us: identify a picture on a quite short description? Nice to share with us. OK my guess: A filter wheel for the world smallest qPCR thermocycler, able to work with nanoliter reactions. Yeah for cheap, but hard to pipette ;)
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby snowcapk » Fri May 09, 2008 4:47 am

White circles = high transmission, so my guess is they are vacuoles or vesicles inside a more electron-dense cell. Mutations in the secretory pathway can cause accumulation of these vesicles (see page three of this pdf).
snowcapk
Garter
Garter
 
Posts: 37
Joined: Fri Oct 26, 2007 2:01 am

Re: interpreting a TEM

Postby cutietech123 » Thu May 29, 2008 11:26 pm

What TEM you mean? Is it Transmission electron microscopy (TEM)? TEM is a microscopy technique whereby a beam of electrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through it.
_____________________

cutie_tech123
I think opinions on wide circles do count.
http://widecirclesworld.wordpress.com
:lol: :roll:
cutietech123
Garter
Garter
 
Posts: 1
Joined: Thu May 29, 2008 11:19 pm

Postby mith » Thu May 29, 2008 11:34 pm

Yes, I'm pretty sure that's what he meant.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
User avatar
mith
Inland Taipan
Inland Taipan
 
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN


Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 1 guest

cron