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glucose transportersModerator: BioTeam
13 posts • Page 2 of 2 • 1, 2
Mr Mistery is right:
-RTqPCR if you have a few genes. Depending on the machines you can multiplex 3 to 5 genes (and that would include a control housekeeping gene to normalize for variations in RT efficiency). The positive side is that you can work with a truckload of samples (say 40 in a 96 wells plates if you include duplicates, controls and normalization curve) everyday (a common program would run in 2 to 3 hours, so you can easilly run 2 to 3 plates a day) -plenty of genes, then it becomes worthy to move to microarrays. But it is usually harder to work with a truckload of samples, since the experiment is time consuming and demands a lot of work. Someone I know was considering 6 samples a day a ggod result. But 100's of gene per sample. There is a trade-off. Unless you can afford robots to do the work. Patrick
Science has proof without any certainty. Creationists have certainty without any proof. (Ashley Montague)
13 posts • Page 2 of 2 • 1, 2
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