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PCR

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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PCR

Postby charanT » Sat Apr 19, 2008 4:54 pm

301 5'gtcagcccca tggtggtggc tggggacagc cacatggtgg tggaggctgg ggtcaaggtg
361 gtagccacag tcagtggaac aagcccagta agccaaaaac caacaygaag catgtggcag
421 gagctgctgc agctggagca gtggtagggg gccttggtgg ctacatgctg ggaagtgcca
481 tgagcaggcc tcttatacat tttggcaatg actatgagga ccgttactat cgtgaaaaca 3'



-A diagram indicating where any primers or probes would anneal to
the above sequence (you can annotate the sequence above).
-A list of any reagents needed, i.e. oligonucleotides, enzymes, etc.
-A statement of how you will detect the polymorphisms
-Controls needed
-The concentrations and formulations of any gels used
-The concentrations and components of any enzymatic reactions used
-A diagram of how the results of your assay would appear including
controls, a homozygous individual and a heterozygote.
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Postby mith » Sat Apr 19, 2008 5:02 pm

I hope there's a text/lecture associated with this.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
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Postby charanT » Sat Apr 19, 2008 11:23 pm

I am not sure what do you mean? this is the problem, mainly I can't figure out the detecting polymorphism, homozygote and hetrozygote?
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Postby snowcapk » Sun Apr 20, 2008 12:05 am

I pity the fool who is heterozygous for sheep prion protein...
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