Identifying an Unkown bacteria.

[nadialindsay]

Hi I need help identifying an unknown bacteria.

According to my gram stain I have a gram negative bacteria that are bacillus shaped. However the other test that I have performed suggest that I may have a Gram positive bacteria. The test are as follow:

Catalases Results are negative
Amalyse results are negative
Bacteria does not digest Casine
TSI: Bacterial growth on agar slant the agar was turned yellow/yellow with no bubbles.
SCA: Bacteria did not catablolize citrate
EMB: There was no bacterial growth
MSA: Bacteria turned the media bright yellow.
Pseud Agar F: Clear color colonies on media
VRB: No colony growth on media
MacConky Agar: No colony growth on media.

I need to identify at least the family name. Any help is appreciated.

[mkwaje]

I could hazard a guess on this one but then I should be the one to get a grade. You can identify this correctly, with a bit of work, using Bergey's Manual of Determinative Bacteriology. I'm pretty sure your library has this. Its much easier to get your answers from the net from other people but its really more satisfactory if you could just spend a little more time with the Bergey's bible. Good luck!

[Sepals]

I advise Cowan and Steel's manual for the identification of medical bacteria. That's what I use.

[Xubu]

why dont you use the Molecular Biology technique in this case? The most accurate method to identify your unknown bacteria is to sequencing its 16S rDNA, i think.

[MrMistery]

That's rRNA, not DNA. And that is really expensive, you know..

[canalon]

That's rRNA, not DNA. And that is really expensive, you know..

No sequencing of the DNA coding for the 16S rRNA. One PCR, and depending of the fragment length 1 or 2 sequencing reaction. Less than 30$ if you do not need to order the primers specifically for that (I assume externalization of sequencing and in lab PCR). So quite cheap in fact.

But I suspect this would problably not help the original poster who was probably trying to figure out the solution to his lab...

[MrMistery]

That might be cheap where you live, not everywhere

[Nad]

That might be cheap where you live, not everywhere

Romania suxs. If a book sells at Amazon with 140 USD, the idiots from Romania inist to sell it with 170 EUR. And so and so and so....

[MrMistery]

Nad, you're from Romania?

[Nad]

Yes, MrMistery, we share this honor of living in Romania