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IgG antibodies

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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IgG antibodies

Postby SmileyScience » Wed Feb 06, 2008 2:11 am

Hi All,

I need to use one of these three antibodies to detect TUBA1 protein in HUVEC cells using western blots:

TUBA1 isotype IgG2b
TUBA1 isotype IgG2a
TUBA1 isotype IgG1

Does anyone know what the main difference between the three IgGs is and which one would you recommend to use?

Regards

Smiley Science
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Re: IgG antibodies

Postby blcr11 » Fri Feb 08, 2008 4:18 pm

I wouldn't have thought that the antibody isotype would make that much of a difference, but I don't do these things, so I may not know what I'm talking about.
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