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Photorespiration

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Photorespiration

Postby negativefx14 » Sat Jan 12, 2008 5:13 pm

Well, for my Biology class, the teacher has assigned us the task of creating an experiment based on photorespiration.

Basically, we create a lab, but dont actually do the experiment.

My problem is, I am unable to think of any good ideas.

I was thinking of a way to express the ratio between oxygen and carbon dioxide, but was unsuccessful :(

If anyone has any good ideas, Id love to hear them.

Thank you :D
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Postby negativefx14 » Sat Jan 12, 2008 9:07 pm

Ok, well, I've been doing more research.

I can have a plant in a stable and controlled atmosphere, where I will be able to control the humidity, temperature, and sunlight the plant recieves. But I am still unable to figure out how to figure out the ratio bewtween the plant using oxygen vs CO2.

I was thnking I could do something with the rubisco enzyme, but im still unable to find a way to calculate the amount of oxygen it uses vs the amount of CO2 it uses in a set amount of time to figure out the ratio.

Grr, this assignment is driving me insane...
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Postby MrMistery » Sat Jan 12, 2008 9:13 pm

try putting it under a glass tube with BaOH as an indicator of the amount of the CO2 in the air.
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Re: Photorespiration

Postby negativefx14 » Sat Jan 12, 2008 9:28 pm

Thank you, thats a good idea.

I will defintly use that. But Im still running into problems with the control groups.

Like doesnt a plant give off co2 and I need to control how much is already in the air, as well as O2...
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Postby mith » Sat Jan 12, 2008 9:30 pm

Since this is design only, you can think of ways to label the atoms of oxygen and carbon dioxide using isotopes.
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Postby negativefx14 » Sat Jan 12, 2008 9:33 pm

Sadly, Im not sure on how to do that.
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Postby negativefx14 » Sat Jan 12, 2008 9:39 pm

Is that a way to distinguish the atmosphere carbon dioxide and oxygen from the ones that you are testing?
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Postby MrMistery » Sat Jan 12, 2008 10:13 pm

yes. a radioactive carbon atom will behave similarly with the regular isotope, but you will be able to identify it through its course in the plant. That is how they figured out photosynthesis initially i think...
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Postby negativefx14 » Sat Jan 12, 2008 10:20 pm

Thank you very much. I think I have enough info to make my lab.


Thanks everyone:)
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Re: Photorespiration

Postby negativefx14 » Sat Jan 12, 2008 11:19 pm

Ive hit another dead end.

For I must be able to collect data from this experiment, and I have no idea how to do this. I cant think of a way to control the test and/or to see the isotopes.

Im literally going insane... I dont know that much about the topic in the first place...
and I keep learning more about it, and it ends up scraping my previous idea...

Would this be a good time to mention im only a high school student, in 11th grade?
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Postby mith » Sun Jan 13, 2008 1:39 am

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Postby mith » Sun Jan 13, 2008 3:52 am

Here's a bit more of a hint,

1. In the overall reaction of photosynthesis, the oxygen used for creating sugars is coming from the carbon dioxide. The oxygen give off as gas is coming from the water.

2. http://www.steve.gb.com/science/photorespiration.html
This link shows a very molecular based set of info about photorespiration.

3. Assume you had normal carbon dioxide and an oxygen isotope in the air. Tracking the products of photorespiration and then measuring the concentrations of air, would you be able to have a sorta accurate rate of photorespiration?
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