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Help with Gene locating

Everything on bioinformatics, the science of information technology as applied to biological research.

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Help with Gene locating

Postby Cye3212 » Tue Dec 11, 2007 3:20 am

You hope to study a gene that codes for a neurotransmitter (NT) protein in human brain cells. You know the amino acid sequence of the protein - how do you: A) identify the genes expressed in a specific type of brain cell, b)ID the gene for the NT, c)produce multiple copies of of the gene, and d) produce a quantity of the NT for evaluation as a potential medication.

any help?? im lost on this one...but if I had to take a guess... for b) look up sequence in worldwide database? im lost on the others... Thanks

-Cye
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Postby mith » Tue Dec 11, 2007 3:53 am

a. Genes produce proteins and also mRNA. If only there was some way to isolate that and amplify+sequence it.

b. You don't need a worldwide database, a human one would work.

c. lookup shotgun sequencing

d. cell cloning.
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Postby canalon » Tue Dec 11, 2007 2:54 pm

A/ RT-PCR and Human DNA chip
B/ what mith said
C/ PCR with primers based on B/
D/ cloning in bacteria or if post transcriptional modifications are needed in another eukaryotic system (yeast, plants or insect cells)
Patrick

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