Biology-Online • View topic - Help with Gene locating

Join for Free!
122463 members

Help with Gene locating

Everything on bioinformatics, the science of information technology as applied to biological research.

Moderator: BioTeam

Help with Gene locating

Postby Cye3212 » Tue Dec 11, 2007 3:20 am

You hope to study a gene that codes for a neurotransmitter (NT) protein in human brain cells. You know the amino acid sequence of the protein - how do you: A) identify the genes expressed in a specific type of brain cell, b)ID the gene for the NT, c)produce multiple copies of of the gene, and d) produce a quantity of the NT for evaluation as a potential medication.

any help?? im lost on this one...but if I had to take a guess... for b) look up sequence in worldwide database? im lost on the others... Thanks

Posts: 1
Joined: Tue Dec 11, 2007 3:16 am

Postby mith » Tue Dec 11, 2007 3:53 am

a. Genes produce proteins and also mRNA. If only there was some way to isolate that and amplify+sequence it.

b. You don't need a worldwide database, a human one would work.

c. lookup shotgun sequencing

d. cell cloning.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
User avatar
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN

Postby canalon » Tue Dec 11, 2007 2:54 pm

A/ RT-PCR and Human DNA chip
B/ what mith said
C/ PCR with primers based on B/
D/ cloning in bacteria or if post transcriptional modifications are needed in another eukaryotic system (yeast, plants or insect cells)

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Return to Bioinformatics

Who is online

Users browsing this forum: No registered users and 1 guest