Login

Join for Free!
114789 members


HELP Failing Biology

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderator: BioTeam

HELP Failing Biology

Postby shell » Fri Nov 30, 2007 4:01 am

:? Please help. I am failing biology and have a homework assignment and cannot find three of the questions. If any one can help I would greatly appreciate it.

1. A biologist isolated a gene from a human cell, attached it to a plasmid, and inserted the plasmid into a bacterium. The plasmid made a new protein, but it was nothing like the protein normally produced in a human cell. Why?

2. Recombinant DNA techniques are used to custom build bacteria for two main purposes: to obtain multiple copies of certain genes and to obtain useful proteins produced by certain genes. Give an example of each of these applications in medicine and agriculture.

3. In the not-too-distant future, gene therapy may be an option for the treatment and cure of some inherited disorders. What do you think are the most serious ethical issues that must be dealt with before human gene therapy is used on a large scale? Why do you think these issues are important?

I know that you guys are not here to do my assignment but I can not figure these three out.

Thanks for any help you can give me.. :)
shell
Garter
Garter
 
Posts: 1
Joined: Fri Nov 30, 2007 3:49 am

Postby mith » Fri Nov 30, 2007 5:46 am

1. no organelles

2.wikipedia

3. will the poor have access?
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
User avatar
mith
Inland Taipan
Inland Taipan
 
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN

Postby AstusAleator » Sat Dec 01, 2007 2:45 am

for number 3, watch the movie Gattaca.
What did the parasitic Candiru fish say when it finally found a host? - - "Urethra!!"
User avatar
AstusAleator
King Cobra
King Cobra
 
Posts: 1039
Joined: Tue Jan 24, 2006 8:51 pm
Location: Oregon, USA


Postby J20gU3 » Sun Dec 02, 2007 3:22 pm

Well for one at least humans have RER and Golgi apparatus not just ribosomes in the cytoplasm. The mere fact of a lack of golgi apparatus would change the protein.
J20gU3
Garter
Garter
 
Posts: 2
Joined: Mon Nov 12, 2007 11:05 pm

Postby MrMistery » Sun Dec 02, 2007 7:37 pm

1. the protein might contain a signal sequence that would have normally been spliced away. Also, it may be due to any posttranslational modifications that might occur in the human cell. However, i think the most obvious differences would come from the presence of introns in human cells.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
User avatar
MrMistery
Inland Taipan
Inland Taipan
 
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)

Postby mith » Sun Dec 02, 2007 8:19 pm

There's also chaperonins etc other folding factors
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
User avatar
mith
Inland Taipan
Inland Taipan
 
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN


Return to Genetics

Who is online

Users browsing this forum: No registered users and 0 guests

cron