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NEED HELP!

Everything on bioinformatics, the science of information technology as applied to biological research.

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NEED HELP!

Postby redondoself » Mon Nov 26, 2007 4:12 pm

Hello, everyone. I am puzzled by a question. If I got a gene in
E.coli, how can I know in which operon it locate? And how can I fix
the mechanism by which its trascripition is regulated?
Thanks a lot in advance.
:(
God help one who helps himself!


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Postby h2so4hurts » Mon Nov 26, 2007 5:01 pm

This sounds like a homework question. I'm pretty sure there are easy tests to determine which major operons regulate your gene... Its probably in your book. Changing the mechanism is pretty easy, just change the operator sequence upstream of the gene. I'm not really sure what the limitations of your answer are though, but there are plenty of solutions.
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Postby mith » Mon Nov 26, 2007 5:20 pm

Microarrays! (That's my answer for everything but it works)
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Enjoying one moment at a time;
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Postby canalon » Tue Nov 27, 2007 3:09 am

Well if you are in E. coli K12(aka MG1655) or O157:H7 you can lookup the genome. Other wise I suggest:
- Make a probe with your gene
- Southern on PFGE profile or BAC Library
- Sequence clone or cloned PFGE fragment
Patrick

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any proof. (Ashley Montague)
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Postby redondoself » Thu Nov 29, 2007 7:11 am

Thank you all very much!
I am sorry for that I did not describe my question clearly enough.
What puzzled me most is how to find the inducer / inhibitor and other components in a pathway and fix their cascade process.
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