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extinction coefficient

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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extinction coefficient

Postby tuphysic » Sun Nov 04, 2007 5:30 pm

Hi
Will somebody guide me how to calculate the extiction coefficient for a dye assembled onto Thin films (Langmuir-Blodgett films, thickness= one monolayer of dye on solid substrate)? Please reply
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Postby pluto » Thu Nov 15, 2007 6:58 am

can you just scrape it off, weigh it, and then measure an absorbance specturm? extinction coefficient is based on the absorance of a 1% solution.
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