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Amino acid sequence

Genetics as it applies to evolution, molecular biology, and medical aspects.

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Postby MrMistery » Tue Oct 30, 2007 12:06 pm

Jessy Springfield wrote:yes, but by manipulation of specific regions?


Ever heard of site-specific induced mutagenesis? It's kind of advanced stuff, but you asked and i will answer. There are several ways to do this, but i'm just gonna explain to you the one I understand is mostly used.
Know what PCR is? If you design your primers so that they will not be 100% the complementaries of those on your DNA molecule but differ in 2-3 bases, they will still align. by designing the primers specifically to place them over the region that you wanna change, you will mutate the DNA exactly at the site you want changed. Then you cut it up with an endonuclease and you insert it into your organism. Easy as 1-2-3. Actually not. Very hard :lol:
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Postby Jessy Springfield » Tue Oct 30, 2007 7:23 pm

MrMistery wrote:
Jessy Springfield wrote:yes, but by manipulation of specific regions?


Ever heard of site-specific induced mutagenesis? It's kind of advanced stuff, but you asked and i will answer. There are several ways to do this, but i'm just gonna explain to you the one I understand is mostly used.
Know what PCR is? If you design your primers so that they will not be 100% the complementaries of those on your DNA molecule but differ in 2-3 bases, they will still align. by designing the primers specifically to place them over the region that you wanna change, you will mutate the DNA exactly at the site you want changed. Then you cut it up with an endonuclease and you insert it into your organism. Easy as 1-2-3. Actually not. Very hard :lol:

Thanks! By any chance do you have any links too information about this? Or information on where too find resources.
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Postby MrMistery » Tue Oct 30, 2007 7:29 pm

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Postby Jessy Springfield » Tue Oct 30, 2007 10:24 pm

MrMistery wrote:ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg.section.611
From Human Molecular Cloning 2


Thanks allot! I appreciate your time.
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Postby MrMistery » Wed Oct 31, 2007 8:21 am

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Postby Cristgonz » Fri Nov 16, 2007 7:07 pm

mith wrote:umm, DNA mutation?

yes, introducing ending codons within the DNA.
for example the UGA one. Thus the transcription will be truncated at the specific place and the other aminoacids wont be expressed.

:shock:
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