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how to get rid off BSA in the medium

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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how to get rid off BSA in the medium

Postby hisunny4u » Mon Oct 01, 2007 12:04 pm

hi every body....
i am culturing Leptospira in EMJH medium containing BSA(very essential)...but leptospira releases Hemolysins...if u try to estimate u get positive result...and one more thing is BSA alone also gives positive result for hemolytic assay my PROBLEM IS HOWCAN I REMOVE BSA FROM THE CULTURE FITRATES(after culturing) that it will b easy for estimation...
BSA is also creating problem while running SDS PAGE is producing a big blob in gel ..which is disturbing the visualization of other bands , i want to get rid off BSA in culture filtrates ...and i cannot even skip as it is essential for it me
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