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Recombinant DNA technology

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Recombinant DNA technology

Postby zami'87. » Sat Jun 04, 2005 10:26 am

ok I know that I was borrrrrrrrrring about theme "protein folding" but couldn't leave it.
I was thinking about synthesis of human proteins by bacteria E. coli So as E. coli is bacterium it has different chaperones than humans so if it would be necessary to produce proteins with quartenary structure that woud ba a problem unless you also insert gene for proper chaperone in E. coli... cause if not the nonfunctional protein could be formed...What do you think? :) I'm just asking for opinion I don't bite you know :lol:
Last edited by zami'87. on Thu Aug 20, 2009 6:17 pm, edited 1 time in total.
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Postby MrMistery » Sat Jun 04, 2005 6:09 pm

Our local expert on E. coli, Canalon will probably have your answer
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E.coli

Postby victor » Sun Jun 05, 2005 12:27 pm

As far as I know that E.coli live inside the human body (I think the place is inside the colon or rectum). They reprocess human's feses and producing K vitamin.[/i]
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Postby MrMistery » Sun Jun 05, 2005 8:41 pm

They live in the last part of the colon if i remember correctly
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Postby zami'87. » Mon Jun 06, 2005 1:20 pm

And why is that important for my topic?I didn't ask that! :?
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Postby Poison » Mon Jun 06, 2005 3:18 pm

zami'87. wrote:And why is that important for my topic?I didn't ask that! :?

It is not. :) The problem is that victor saw the word 'E.Coli' and didn't read further... :lol:
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Postby zami'87. » Mon Jun 06, 2005 3:53 pm

Poison wrote:
zami'87. wrote:And why is that important for my topic?I didn't ask that! :?

It is not. :) The problem is that victor saw the word 'E.Coli' and didn't read further... :lol:


sorry.I wasn't angry. I just don't want to rename topic.
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Postby canalon » Mon Jun 06, 2005 5:34 pm

The answer from the local E. coli expert is that he doesn't play with those eucaryotic genes. And he wouldn't contaminate his nice pet bacteria with such horrors. 8)

More seriously, it is indeed true that you cannot express all eucaryotic genes in bacteria. And that is exacly why you have so many diffrent eucaryotic expression systems (from insect cells to GM plants). And inserting just the proper chaaperone is just not enough, or you would have the strain going around if it was so simple. There are a lot of post translationnal modification that cannot be processed in bacteria.

Hope this helps (not lazy today, rather trying to avoid helping in the lab clean-up)

Patrick
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Postby zami'87. » Mon Jun 06, 2005 5:49 pm

Canalon wrote:The answer from the local E. coli expert is that he doesn't play with those eucaryotic genes. And he wouldn't contaminate his nice pet bacteria with such horrors. 8)

More seriously, it is indeed true that you cannot express all eucaryotic genes in bacteria. And that is exacly why you have so many diffrent eucaryotic expression systems (from insect cells to GM plants). And inserting just the proper chaaperone is just not enough, or you would have the strain going around if it was so simple. There are a lot of post translationnal modification that cannot be processed in bacteria.

Hope this helps (not lazy today, rather trying to avoid helping in the lab clean-up)

Patrick

Thanks Patrick :D !
I know it's still a secret but in the future.....
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Postby chemistry_freako » Mon Jul 04, 2005 3:14 pm

Hmm oh - then how about:
EHEC
ETEC
EIEC
EPEC? =D just wanna know more :shock:
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Postby canalon » Tue Jul 05, 2005 1:38 am

chemistry_freako wrote:Hmm oh - then how about:
EHEC
ETEC
EIEC
EPEC? =D just wanna know more :shock:

The final EC stands for Escherichi coli as for the rest:
EH: EnteroHaemoragic
ET: Entero toxigenic
EI: Entero invasive
EP: Enteropathogenic

These are different type of pathogenic E. coli, it is a medical classification that reflects the main set of virulence genes in the bacteria and hence it's mode of infection. I think ther were more, but i do not find my references books to complete your list.

But again, what's the relation with the topic?

Patrick[/i]
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