Recombinant DNA technology

[zami'87.]

ok I know that I was borrrrrrrrrring about theme "protein folding" but couldn't leave it.
I was thinking about synthesis of human proteins by bacteria E. coli So as E. coli is bacterium it has different chaperones than humans so if it would be necessary to produce proteins with quartenary structure that woud ba a problem unless you also insert gene for proper chaperone in E. coli... cause if not the nonfunctional protein could be formed...What do you think? I'm just asking for opinion I don't bite you know

[MrMistery]

Our local expert on E. coli, Canalon will probably have your answer

[victor]

As far as I know that E.coli live inside the human body (I think the place is inside the colon or rectum). They reprocess human's feses and producing K vitamin.[/i]

[MrMistery]

They live in the last part of the colon if i remember correctly

[zami'87.]

And why is that important for my topic?I didn't ask that!

[Poison]

And why is that important for my topic?I didn't ask that!

It is not. The problem is that victor saw the word 'E.Coli' and didn't read further...

[zami'87.]

And why is that important for my topic?I didn't ask that!

It is not. The problem is that victor saw the word 'E.Coli' and didn't read further...

sorry.I wasn't angry. I just don't want to rename topic.

[canalon]

The answer from the local E. coli expert is that he doesn't play with those eucaryotic genes. And he wouldn't contaminate his nice pet bacteria with such horrors.

More seriously, it is indeed true that you cannot express all eucaryotic genes in bacteria. And that is exacly why you have so many diffrent eucaryotic expression systems (from insect cells to GM plants). And inserting just the proper chaaperone is just not enough, or you would have the strain going around if it was so simple. There are a lot of post translationnal modification that cannot be processed in bacteria.

Hope this helps (not lazy today, rather trying to avoid helping in the lab clean-up)

Patrick

[zami'87.]

The answer from the local E. coli expert is that he doesn't play with those eucaryotic genes. And he wouldn't contaminate his nice pet bacteria with such horrors.

More seriously, it is indeed true that you cannot express all eucaryotic genes in bacteria. And that is exacly why you have so many diffrent eucaryotic expression systems (from insect cells to GM plants). And inserting just the proper chaaperone is just not enough, or you would have the strain going around if it was so simple. There are a lot of post translationnal modification that cannot be processed in bacteria.

Hope this helps (not lazy today, rather trying to avoid helping in the lab clean-up)

Patrick

Thanks Patrick !
I know it's still a secret but in the future.....

[chemistry_freako]

Hmm oh - then how about:
EHEC
ETEC
EIEC
EPEC? =D just wanna know more

[canalon]

Hmm oh - then how about:
EHEC
ETEC
EIEC
EPEC? =D just wanna know more

The final EC stands for Escherichi coli as for the rest:
EH: EnteroHaemoragic
ET: Entero toxigenic
EI: Entero invasive
EP: Enteropathogenic

These are different type of pathogenic E. coli, it is a medical classification that reflects the main set of virulence genes in the bacteria and hence it's mode of infection. I think ther were more, but i do not find my references books to complete your list.

But again, what's the relation with the topic?

Patrick[/i]