Clumping of BHK21 cells with trypsin

[manuli]

I am using rodent cell line BHK21. They clump very badly when you trypsinize them. It becomes to count and seed.

I use DMEM 10 % FCS with 1X non-essential amino acids to grow the cells.
Does any one have any ideas regarding resolving the clumping problem??????

[Beaker73]

Perhaps a syringe with an 18g drawing up needle. Drawing the suspension up and down gently a few times often disperses clumps.