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HELP ME PLEASE ......!!

Debate and discussion of any biological questions not pertaining to a particular topic.

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HELP ME PLEASE ......!!

Postby BCH04 » Sun Jun 17, 2007 6:41 pm

I have this take home test and its due 2morrow in my microbiology class

and its my 2nd time taking this class . I was wondering if some one could

go over these questions just to see if I did good!!!



TEST

1. Which of the following compunds has the gratest amount of chemical energy
a.ATP
b.NADH
C.FADH
D. glucose
E.Coenzyme A

2.which organism is not correctly matched to its energy source?
a.photoheterotroph- light
b.photoautotroph-carbon dioxide
c.chemoautotroph- Fe2+
d.chemoheterotroph-glucose
e.chemoautotroph - Nh3

3. Which of the following statements about aerobic respiration is false ?
a. It involves glycolysis only
b. it involves the Krebs cycle
c. It generates NADH and FADH
d. it generates ATP
e. It requires cytochromes

4. Which of the following processes does not genetae ATP?
A. Photophosphorylation
B. Calvin - Benson cycle
C. oxidative phosphorylation
D. substrate - level phosphorylation

5. How man molecules of ATP can be generated from the complete oxidation of glucose to CO2 and H2O in a typical prokaryotic cell?
a. 2
b. 4
c 34
d. 36
e. 38

6. Which of the following is the best definition of respiration?
A. a sequence of carrier molecules with O2 as the final electron acceptor
b . a sequence of carrier molecules with an inorganice molecule as the final electron acceptor
c. a method of generating ATP
d. the complete oxidation of glucose to CO2 and H2O
E. a series of reactions in which pyruvic acid is oxidized to CO2 and H2O

7. If glucose were completely oxidized to carbon dioxide and water, yielding the maximum amount of ATP, approximately what percentage of the ATP would be generated via aerobic respiration?
A. 0
B. 25
C. 50
D .89
E. 100

8. According to the chemiosmotic mechanism, ATP is genreated when
A. electrons are transferred between carrier molecules
B. a high energy phosphate group is transferred from an intermediate metabolite to ADP
C. chlorophyll liberates an electron
D. protons are moved across a membrane
E. cells lyse is a hypotonic environment

9. All of the following are true about substrace- level phosphorylation Except
a. it involves direct transfer of a highe energy phosphate group from a compund to ATP
b. No final electron acceptor is required
C. it occurs in glycolysis
d. oxidation of intermediate metabolic compunds relesase energy that is used to generate ATP.
E. all of the above stastements are true

10. Which of the following is the best defination of fermentation?
A. the reduction of glucose pyruvic acid
b. the oxidation of glucose with organic molecules serving as electron carriers
c. the complete catabolism of glucose to CO2 and H2O
D. the production of energy by substrate- level phosphorylation
e. the production of ethyl alcohol from glucose


use the following answers for questions 18-21. Each answer may be used once , more than once or not al all
A. Escherichia coli growing in glucose broth at 35 C with oxygen (O2)
B. Escherichia coli growing in glucose broth at 35 C without oxygen ( O2)
C. Both A and B
D. neither A nor B

11. Which culture produces the most E. Coli
12.Which culture produces the most lactic acid
13. which culture uses NAD?
14.Which culture produces the most ATP

-------------------------------------------------

15. Which of the following pathways may be used with glycolysis to produce ribose?
a. Entner- Doudoroff pathway
b. pentose- phophate pahtway
c. Krebs-cycle
d. Aan B only
E. A,B,and C

16. Which pathway below is used by some bacteria instead of glycolysis?
A. entmer- doudoroff pathway
b. krebs- cycle
c. pentose- phosphate pathway
d. all of the above
e. none of the above

17.whcich statement regarding metabolism is true?
a. catabolic reactions produce energy.
b. anabolic reactions require energy
c. vitamins are used as coenzymes in enzymatic reactions.
d. all of the above
e. none of the above

18. Feedback inhibition
a. usually affects the last enzyme in a metabolic pathway
b. is a form of competitive inhibition
c. causes the energy of activation to be lowered
d. usually results when the pathway product is in excess
e. none of the above

19.which process below is characterized as anabolic
a. Protein syntheses
B. fermentation
c. lactic acid production
d. anaerobic respiration
e. aerobic respirtaion

20. The addition of which of the following to a culture medium will neutralize bases?
a. buffers
b. sugars.
c. pH
d. heat
e. carbon

21. salts ans sugars work to preserve food by creating
a. a depletion of nutrients
b. a hypotonic environment
c. lower osmotic pressure
d. a hypertonic environment
e. lower pH

22. the term faculative anaerobe refers to an organism that
a. does not use oxy gen but tolerates it
b. is killded by oxugen
c. uses oxygen or grows without oxygen
d. requires less oxygen than is present in air
e. prefers to grow without oxygen

23. the term trace elements refers to
a. the elements represented by the acronym CHONPS
b. vitamins
c. nitrogen, phosphorus and sulfur
d. small minerl requirements
e. toxic substances

24. which type of bacterium would be most likely killed in the presence of oxygen?
a. strict aerobe
b. strict anarobe
c. facultative anaerobe
d. aerotolerant anarobe
e. microaerophille

25. which of the following tupes of media would be used to culture anaerobes?
A. Selective media
b. reducing media
c. enrichment media
d. differential media
e. complex media

26. all of the following are true about agar except
a. it is a source of nutirents in culture media.
b. it is a polysaccharide
c. it liquefies at 100 c
D. it solidifies at approximately 40 C
e. IT cannot be broken down by most bacteria

27. 36 colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard p;ate count. How many cells were in the orginal sample?
A. 4 per ml
b. 9 per ml
c. 18 per ml
d.36 per ml
e. 72 per ml

28. most bacteria gorw best at pH
a. 1
b. 5
c. 7
d.9
e.14

29. which of the following is the best definition of generation time?
A. the length of time it takes for lag phase
b. the length of time it takes for a cell to divide
c. the minimun rate of doubling
d. the duration of log phase
e. the time it take for nuclear division

30. A culture medium on which only Gram-positive organisms grown and a yellow halo surrounds only staphylococcus aureus colonies is called a
a. selective medium
b. differential medium
c. enrichment medium
d. A and B
E. B and C

31.A gene is Best defined as
a. a sequence of DNA
b. 3 nucleotides that code for an amino acid
c. a sequence of nucleotides in DNA that codes for a functional product
d. a sequence of nucleotides in RNA that codes for a functional product
E. a transcribed un it of DNA

32.Which of the following pairs is MISMATCHED?
A. DNA polymerase- makes a molecule of DNA from a DNA template
B.. RNA polymerase- makes a molecule of RNA from an RNA template
C. DNA- ligase - joins segments of DNA
D.transpose - insertion of DNA segments into DNA
e. Spliceosome- removal of inrons

33. Genetoc change in bacteria can be brought about by
a. Mutation
B. conjugation
C. transductions
D. transformation
e. all of the above

34. which o the following enzymes is not found in any organism?
A. DNA polymerase
B. RNA polymerase
c. reverse transcriptase
d. ribozymes
e. DNA ligase

35. During replication and transcription
a. DNA is " read" in a 3 to 5 direction
b/ DNA is manufaactured in a 5 to 3 direction
c. the bases added in both processes are deoxyribonucleotides
d. a and b only
e. A, B and C

36. True or False ' The bacterial chromosome is a doube stranded linear molecule"
T or F

37.Before replication takes place in bacteria, the chromosomes is normally
a. a large relaxed covalently closed circle
b. linear
c. a highly compact and supercoilded double stranded circle
d. found inside the nucleus of the cell
e. covered with ribosomes

38. which of the following staements is FALSE
a. DNA polymerase jois nucleotides in the 3 ---->5 direction only
b. the leading strand of DNA is made continous;y
c. the lagging strand of DNA is started by and RNA primer
d. DNA replication proceeds in one direction around the bacterial chromosome
e. multiple replication forks are possible on a bacterial chromose

39.. The transfer of DNA from a donor to a recipient cell by a bacteriophage is
a. conjugartion
b. transduction
c. transformation
d. genetic engineering
e. none of the above

40. which of the follwoing is NOT a product of transcription?
a. a new strand of DNA
b. rRNA
c.tRNA
d.mRNA
e. none of the above

41. an enzyme produced in response to the presence of a substrate is called
a. an inducible enzyme
b. a repressible enzyme
c. a restriction enzyme
d. an operator
e. a promoter

42. The DNA probe, GGCTTA, will hybridize with DNA containing
A. CCGUUA
B.CCGAAT
C.GGCTTA
D.GGUUA
E.GGCAAU

43. Which of the following is the reaction catalyzed by DNA - dependent RNA polymerase?
A. DNA----> mRNA
B. mRNA---> cDNA
C.mRNA---> protein
D. DNA ---> DNA
e. none of the above

44. Which of the following enzymes is used in replication of the lagging DNA strand?
A. RNA polymerase
B. DNA polymerase
C. DNA ligase
D. A and B only
E. A,B and C

45. In transcription
a. DNA is change to RNA
b. DNA is copied to RNA
c. DNA is replicated
d. RNA is copied to DNA
e. proteins are made

46. the value of cDNA in genetic engineering is that
a. it lacks exons
b. it lacks introns
c. it is really RNA
d. it allows prokaryotic genes to be inserted into eukaryotic dna
e. none of the above

USE THE FOLLOWING INFO TO ANSWER QUESTIONS 47-49
CONDON ON mRNA and the corresponding amino acid

47. if the sequence of amino acids cpded for by a strand of DNA is valine- histidine proline arginine what is the order of bases in the strand of DNA?
A CAGGTAGACTAC
B. GUUCAUCCGCGU
C.CAAGTAGGCGCA
D.GACGTAACATTT
E.CAGGGCGGCGCA

The antisense ( complimentary) strand coding for the polypeptide in question 47 is
A. GTCCATCTGATG
B.GTTCATCCGCGT
C.GUUCAUCCGCGT
D.GTCCCGCCGCGT
E.GTCCATCTGATG

What os the sequence of mino acids coded for by the following sequence of bases in a strand of DNA? GACTACACATTT
A. Leucine methionine cysteine lysine
B. prline arginine histidine proline
c. leucine lysine arginine valine
d. transcription would stop at the first codon
e. cannont be determined from the data given

What is the anticodon for methionine?
a. TAC
B. GTA
C. AUG
D. CGA
E. UAC

which of the following is not used in make cDNA?
A. REVERSE TRANCRIPTION
B. RNA processing to remove introns
c. transcription
d, translation
e. i do not know, so I am choosing this wrong answer





IM BEGGING , ASKING IF SOME ONE COULD GO OVER SO I CAN MAKE MY CORRECTIONS
Last edited by BCH04 on Mon Jun 18, 2007 4:15 pm, edited 1 time in total.
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Postby Tae Jun, Yoon » Mon Jun 18, 2007 8:52 am

I think this kind of posts have to be DELETED by administrator :evil:
What makes the desert beautiful
is that somewhere it hide a well - The Little Prince
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Postby Poison » Mon Jun 18, 2007 1:09 pm

Where, in this site, does it write: "We do your homework for free!!!" Nowhere....
At least TRY to do it yourself.
It matters not how strait the gate
How charged with punishment the scroll
I am the Master of my fate
I am the Captain of my soul.
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Postby mehdi71000 » Mon Jun 18, 2007 1:55 pm

Is this sticky? The whole point of school is learning. If you like copying take a typing class. You can do it man just understand the topic and your smiling
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Postby blcr11 » Mon Jun 18, 2007 1:58 pm

Caveat emptor.
________________________________________
I have this take home test and its due 2morrow in my microbiology class

and its my 2nd time taking this class . I was wondering if some one could

go over these questions just to see if I did good!!!



TEST

1. Which of the following compunds has the gratest amount of chemical energy A
a.ATP
b.NADH
C.FADH
D. glucose
E.Coenzyme A

2.which organism is not correctly matched to its energy source? Not familiar with terms & I’m too lazy to look them up
a.photoheterotroph- light
b.photoautotroph-carbon dioxide
c.chemoautotroph- Fe2+
d.chemoheterotroph-glucose
e.chemoautotroph - Nh3

3. Which of the following statements about aerobic respiration is false ? A
a. It involves glycolysis only
b. it involves the Krebs cycle
c. It generates NADH and FADH
d. it generates ATP
e. It requires cytochromes

4. Which of the following processes does not genetae ATP? B
A. Photophosphorylation
B. Calvin - Benson cycle
C. oxidative phosphorylation
D. substrate - level phosphorylation

5. How man molecules of ATP can be generated from the complete oxidation of glucose to CO2 and H2O in a typical prokaryotic cell? E
a. 2
b. 4
c 34
d. 36
e. 38

6. Which of the following is the best definition of respiration? D
A. a sequence of carrier molecules with O2 as the final electron acceptor
b . a sequence of carrier molecules with an inorganice molecule as the final electron acceptor
c. a method of generating ATP
d. the complete oxidation of glucose to CO2 and H2O
E. a series of reactions in which pyruvic acid is oxidized to CO2 and H2O

7. If glucose were completely oxidized to carbon dioxide and water, yielding the maximum amount of ATP, approximately what percentage of the ATP would be generated via aerobic respiration? D (a guess)
A. 0
B. 25
C. 50
D .89
E. 100

8. According to the chemiosmotic mechanism, ATP is genreated when D
A. electrons are transferred between carrier molecules
B. a high energy phosphate group is transferred from an intermediate metabolite to ADP
C. chlorophyll liberates an electron
D. protons are moved across a membrane
E. cells lyse is a hypotonic environment

9. All of the following are true about substrace- level phosphorylation Except E (although possibly D – would have to check & I’m too lazy to look it up)
a. it involves direct transfer of a highe energy phosphate group from a compund to ATP
b. No final electron acceptor is required
C. it occurs in glycolysis
d. oxidation of intermediate metabolic compunds relesase energy that is used to generate ATP.
E. all of the above stastements are true

10. Which of the following is the best defination of fermentation? E
A. the reduction of glucose pyruvic acid
b. the oxidation of glucose with organic molecules serving as electron carriers
c. the complete catabolism of glucose to CO2 and H2O
D. the production of energy by substrate- level phosphorylation
e. the production of ethyl alcohol from glucose


use the following answers for questions 18-21. Each answer may be used once , more than once or not al all
A. Escherichia coli growing in glucose broth at 35 C with oxygen (O2)
B. Escherichia coli growing in glucose broth at 35 C without oxygen ( O2)
C. Both A and B
D. neither A nor B

11. Which culture produces the most E. Coli A
12.Which culture produces the most lactic acid B
13. which culture uses NAD? (Poor question, but I’d guess C)
14.Which culture produces the most ATP A

-------------------------------------------------

15. Which of the following pathways may be used with glycolysis to produce ribose? B – again I’d have to check to be sure
a. Entner- Doudoroff pathway
b. pentose- phophate pahtway
c. Krebs-cycle
d. Aan B only
E. A,B,and C

16. Which pathway below is used by some bacteria instead of glycolysis? D
A. entmer- doudoroff pathway
b. krebs- cycle
c. pentose- phosphate pathway
d. all of the above
e. none of the above

17.whcich statement regarding metabolism is true? D
a. catabolic reactions produce energy.
b. anabolic reactions require energy
c. vitamins are used as coenzymes in enzymatic reactions.
d. all of the above
e. none of the above

18. Feedback inhibition - not sure, I think D
a. usually affects the last enzyme in a metabolic pathway
b. is a form of competitive inhibition
c. causes the energy of activation to be lowered
d. usually results when the pathway product is in excess
e. none of the above

19.which process below is characterized as anabolic A
a. Protein syntheses
B. fermentation
c. lactic acid production
d. anaerobic respiration
e. aerobic respirtaion

20. The addition of which of the following to a culture medium will neutralize bases? A, but poor choice of words
a. buffers
b. sugars.
c. pH
d. heat
e. carbon

21. salts ans sugars work to preserve food by creating D
a. a depletion of nutrients
b. a hypotonic environment
c. lower osmotic pressure
d. a hypertonic environment
e. lower pH

22. the term faculative anaerobe refers to an organism that C
a. does not use oxy gen but tolerates it
b. is killded by oxugen
c. uses oxygen or grows without oxygen
d. requires less oxygen than is present in air
e. prefers to grow without oxygen

23. the term trace elements refers to D?
a. the elements represented by the acronym CHONPS
b. vitamins
c. nitrogen, phosphorus and sulfur
d. small minerl requirements
e. toxic substances

24. which type of bacterium would be most likely killed in the presence of oxygen? B
a. strict aerobe
b. strict anarobe
c. facultative anaerobe
d. aerotolerant anarobe
e. microaerophille

25. which of the following tupes of media would be used to culture anaerobes? B
A. Selective media
b. reducing media
c. enrichment media
d. differential media
e. complex media

26. all of the following are true about agar except A
a. it is a source of nutirents in culture media.
b. it is a polysaccharide
c. it liquefies at 100 c
D. it solidifies at approximately 40 C
e. IT cannot be broken down by most bacteria

27. 36 colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard p;ate count. How many cells were in the orginal sample? C—must be a very slow grower
A. 4 per ml
b. 9 per ml
c. 18 per ml
d.36 per ml
e. 72 per ml

28. most bacteria gorw best at pH C
a. 1
b. 5
c. 7
d.9
e.14

29. which of the following is the best definition of generation time? B
A. the length of time it takes for lag phase
b. the length of time it takes for a cell to divide
c. the minimun rate of doubling
d. the duration of log phase
e. the time it take for nuclear division

30. A culture medium on which only Gram-positive organisms grown and a yellow halo surrounds only staphylococcus aureus colonies is called a - Not sure guessing D
a. selective medium
b. differential medium
c. enrichment medium
d. A and B
E. B and C

31.A gene is Best defined as C
a. a sequence of DNA
b. 3 nucleotides that code for an amino acid
c. a sequence of nucleotides in DNA that codes for a functional product
d. a sequence of nucleotides in RNA that codes for a functional product
E. a transcribed un it of DNA

32.Which of the following pairs is MISMATCHED? B, I suppose
A. DNA polymerase- makes a molecule of DNA from a DNA template
B.. RNA polymerase- makes a molecule of RNA from an RNA template
C. DNA- ligase - joins segments of DNA
D.transpose - insertion of DNA segments into DNA
e. Spliceosome- removal of inrons

33. Genetoc change in bacteria can be brought about by E
a. Mutation
B. conjugation
C. transductions
D. transformation
e. all of the above

34. which o the following enzymes is not found in any organism? C on a technicality
A. DNA polymerase
B. RNA polymerase
c. reverse transcriptase
d. ribozymes
e. DNA ligase

35. During replication and transcription A
a. DNA is " read" in a 3 to 5 direction
b/ DNA is manufaactured in a 5 to 3 direction
c. the bases added in both processes are deoxyribonucleotides
d. a and b only
e. A, B and C

36. True or False ' The bacterial chromosome is a doube stranded linear molecule"
T or F False – most (all?) have circular chromosomes

37.Before replication takes place in bacteria, the chromosomes is normally C
a. a large relaxed covalently closed circle
b. linear
c. a highly compact and supercoilded double stranded circle
d. found inside the nucleus of the cell
e. covered with ribosomes

38. which of the following staements is FALSE D
a. DNA polymerase jois nucleotides in the 3 ---->5 direction only
b. the leading strand of DNA is made continous;y
c. the lagging strand of DNA is started by and RNA primer
d. DNA replication proceeds in one direction around the bacterial chromosome
e. multiple replication forks are possible on a bacterial chromose

39.. The transfer of DNA from a donor to a recipient cell by a bacteriophage is B
a. conjugartion
b. transduction
c. transformation
d. genetic engineering
e. none of the above

40. which of the follwoing is NOT a product of transcription? A
a. a new strand of DNA
b. rRNA
c.tRNA
d.mRNA
e. none of the above

41. an enzyme produced in response to the presence of a substrate is called A
a. an inducible enzyme
b. a repressible enzyme
c. a restriction enzyme
d. an operator
e. a promoter

42. The DNA probe, GGCTTA, will hybridize with DNA containing – most likely C, but the polarity of all strands should be given in order to answer the question properly
A. CCGUUA
B.CCGAAT
C.GGCTTA
D.GGUUA
E.GGCAAU

43. Which of the following is the reaction catalyzed by DNA - dependent RNA polymerase?
A. DNA----> mRNA
B. mRNA---> cDNA
C.mRNA---> protein
D. DNA ---> DNA
e. none of the above

44. Which of the following enzymes is used in replication of the lagging DNA strand? – Not sure, have to look it up possibly E?
A. RNA polymerase
B. DNA polymerase
C. DNA ligase
D. A and B only
E. A,B and C

45. In transcription B
a. DNA is change to RNA
b. DNA is copied to RNA
c. DNA is replicated
d. RNA is copied to DNA
e. proteins are made

46. the value of cDNA in genetic engineering is that B?
a. it lacks exons
b. it lacks introns
c. it is really RNA
d. it allows prokaryotic genes to be inserted into eukaryotic dna
e. none of the above

USE THE FOLLOWING INFO TO ANSWER QUESTIONS 47-49 - requires a codon table & I’m too lazy to look one up or use one – on your own for those
CONDON ON mRNA and the corresponding amino acid

47. if the sequence of amino acids cpded for by a strand of DNA is valine- histidine proline arginine what is the order of bases in the strand of DNA?
A CAGGTAGACTAC
B. GUUCAUCCGCGU
C.CAAGTAGGCGCA
D.GACGTAACATTT
E.CAGGGCGGCGCA

The antisense ( complimentary) strand coding for the polypeptide in question 47 is
A. GTCCATCTGATG
B.GTTCATCCGCGT
C.GUUCAUCCGCGT
D.GTCCCGCCGCGT
E.GTCCATCTGATG

What os the sequence of mino acids coded for by the following sequence of bases in a strand of DNA? GACTACACATTT
A. Leucine methionine cysteine lysine
B. prline arginine histidine proline
c. leucine lysine arginine valine
d. transcription would stop at the first codon
e. cannont be determined from the data given

What is the anticodon for methionine?
a. TAC
B. GTA
C. AUG
D. CGA
E. UAC

which of the following is not used in make cDNA? D
A. REVERSE TRANCRIPTION
B. RNA processing to remove introns
c. transcription
d, translation
e. i do not know, so I am choosing this wrong answer

Took me all of 20 minutes to do that without a text and I make to claims to know what I'm talking about. It would have taken a little longer if I had bothered to look up a few things and to read things off a codon table. If I'm wrong and you copy down my wrong answer, too bad. See, the point is to learn something, not just to take the bleeping test. - As I see others are trying to say as well.
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Postby blcr11 » Mon Jun 18, 2007 2:06 pm

That should be...I make no claim to know what I'm talking about...
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Postby BCH04 » Mon Jun 18, 2007 4:15 pm

blcr11 wrote:Caveat emptor.
________________________________________
I have this take home test and its due 2morrow in my microbiology class

and its my 2nd time taking this class . I was wondering if some one could

go over these questions just to see if I did good!!!



TEST

1. Which of the following compunds has the gratest amount of chemical energy A
a.ATP
b.NADH
C.FADH
D. glucose
E.Coenzyme A

2.which organism is not correctly matched to its energy source? Not familiar with terms & I’m too lazy to look them up
a.photoheterotroph- light
b.photoautotroph-carbon dioxide
c.chemoautotroph- Fe2+
d.chemoheterotroph-glucose
e.chemoautotroph - Nh3

3. Which of the following statements about aerobic respiration is false ? A
a. It involves glycolysis only
b. it involves the Krebs cycle
c. It generates NADH and FADH
d. it generates ATP
e. It requires cytochromes

4. Which of the following processes does not genetae ATP? B
A. Photophosphorylation
B. Calvin - Benson cycle
C. oxidative phosphorylation
D. substrate - level phosphorylation

5. How man molecules of ATP can be generated from the complete oxidation of glucose to CO2 and H2O in a typical prokaryotic cell? E
a. 2
b. 4
c 34
d. 36
e. 38

6. Which of the following is the best definition of respiration? D
A. a sequence of carrier molecules with O2 as the final electron acceptor
b . a sequence of carrier molecules with an inorganice molecule as the final electron acceptor
c. a method of generating ATP
d. the complete oxidation of glucose to CO2 and H2O
E. a series of reactions in which pyruvic acid is oxidized to CO2 and H2O

7. If glucose were completely oxidized to carbon dioxide and water, yielding the maximum amount of ATP, approximately what percentage of the ATP would be generated via aerobic respiration? D (a guess)
A. 0
B. 25
C. 50
D .89
E. 100

8. According to the chemiosmotic mechanism, ATP is genreated when D
A. electrons are transferred between carrier molecules
B. a high energy phosphate group is transferred from an intermediate metabolite to ADP
C. chlorophyll liberates an electron
D. protons are moved across a membrane
E. cells lyse is a hypotonic environment

9. All of the following are true about substrace- level phosphorylation Except E (although possibly D – would have to check & I’m too lazy to look it up)
a. it involves direct transfer of a highe energy phosphate group from a compund to ATP
b. No final electron acceptor is required
C. it occurs in glycolysis
d. oxidation of intermediate metabolic compunds relesase energy that is used to generate ATP.
E. all of the above stastements are true

10. Which of the following is the best defination of fermentation? E
A. the reduction of glucose pyruvic acid
b. the oxidation of glucose with organic molecules serving as electron carriers
c. the complete catabolism of glucose to CO2 and H2O
D. the production of energy by substrate- level phosphorylation
e. the production of ethyl alcohol from glucose


use the following answers for questions 18-21. Each answer may be used once , more than once or not al all
A. Escherichia coli growing in glucose broth at 35 C with oxygen (O2)
B. Escherichia coli growing in glucose broth at 35 C without oxygen ( O2)
C. Both A and B
D. neither A nor B

11. Which culture produces the most E. Coli A
12.Which culture produces the most lactic acid B
13. which culture uses NAD? (Poor question, but I’d guess C)
14.Which culture produces the most ATP A

-------------------------------------------------

15. Which of the following pathways may be used with glycolysis to produce ribose? B – again I’d have to check to be sure
a. Entner- Doudoroff pathway
b. pentose- phophate pahtway
c. Krebs-cycle
d. Aan B only
E. A,B,and C

16. Which pathway below is used by some bacteria instead of glycolysis? D
A. entmer- doudoroff pathway
b. krebs- cycle
c. pentose- phosphate pathway
d. all of the above
e. none of the above

17.whcich statement regarding metabolism is true? D
a. catabolic reactions produce energy.
b. anabolic reactions require energy
c. vitamins are used as coenzymes in enzymatic reactions.
d. all of the above
e. none of the above

18. Feedback inhibition - not sure, I think D
a. usually affects the last enzyme in a metabolic pathway
b. is a form of competitive inhibition
c. causes the energy of activation to be lowered
d. usually results when the pathway product is in excess
e. none of the above

19.which process below is characterized as anabolic A
a. Protein syntheses
B. fermentation
c. lactic acid production
d. anaerobic respiration
e. aerobic respirtaion

20. The addition of which of the following to a culture medium will neutralize bases? A, but poor choice of words
a. buffers
b. sugars.
c. pH
d. heat
e. carbon

21. salts ans sugars work to preserve food by creating D
a. a depletion of nutrients
b. a hypotonic environment
c. lower osmotic pressure
d. a hypertonic environment
e. lower pH

22. the term faculative anaerobe refers to an organism that C
a. does not use oxy gen but tolerates it
b. is killded by oxugen
c. uses oxygen or grows without oxygen
d. requires less oxygen than is present in air
e. prefers to grow without oxygen

23. the term trace elements refers to D?
a. the elements represented by the acronym CHONPS
b. vitamins
c. nitrogen, phosphorus and sulfur
d. small minerl requirements
e. toxic substances

24. which type of bacterium would be most likely killed in the presence of oxygen? B
a. strict aerobe
b. strict anarobe
c. facultative anaerobe
d. aerotolerant anarobe
e. microaerophille

25. which of the following tupes of media would be used to culture anaerobes? B
A. Selective media
b. reducing media
c. enrichment media
d. differential media
e. complex media

26. all of the following are true about agar except A
a. it is a source of nutirents in culture media.
b. it is a polysaccharide
c. it liquefies at 100 c
D. it solidifies at approximately 40 C
e. IT cannot be broken down by most bacteria

27. 36 colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard p;ate count. How many cells were in the orginal sample? C—must be a very slow grower
A. 4 per ml
b. 9 per ml
c. 18 per ml
d.36 per ml
e. 72 per ml

28. most bacteria gorw best at pH C
a. 1
b. 5
c. 7
d.9
e.14

29. which of the following is the best definition of generation time? B
A. the length of time it takes for lag phase
b. the length of time it takes for a cell to divide
c. the minimun rate of doubling
d. the duration of log phase
e. the time it take for nuclear division

30. A culture medium on which only Gram-positive organisms grown and a yellow halo surrounds only staphylococcus aureus colonies is called a - Not sure guessing D
a. selective medium
b. differential medium
c. enrichment medium
d. A and B
E. B and C

31.A gene is Best defined as C
a. a sequence of DNA
b. 3 nucleotides that code for an amino acid
c. a sequence of nucleotides in DNA that codes for a functional product
d. a sequence of nucleotides in RNA that codes for a functional product
E. a transcribed un it of DNA

32.Which of the following pairs is MISMATCHED? B, I suppose
A. DNA polymerase- makes a molecule of DNA from a DNA template
B.. RNA polymerase- makes a molecule of RNA from an RNA template
C. DNA- ligase - joins segments of DNA
D.transpose - insertion of DNA segments into DNA
e. Spliceosome- removal of inrons

33. Genetoc change in bacteria can be brought about by E
a. Mutation
B. conjugation
C. transductions
D. transformation
e. all of the above

34. which o the following enzymes is not found in any organism? C on a technicality
A. DNA polymerase
B. RNA polymerase
c. reverse transcriptase
d. ribozymes
e. DNA ligase

35. During replication and transcription A
a. DNA is " read" in a 3 to 5 direction
b/ DNA is manufaactured in a 5 to 3 direction
c. the bases added in both processes are deoxyribonucleotides
d. a and b only
e. A, B and C

36. True or False ' The bacterial chromosome is a doube stranded linear molecule"
T or F False – most (all?) have circular chromosomes

37.Before replication takes place in bacteria, the chromosomes is normally C
a. a large relaxed covalently closed circle
b. linear
c. a highly compact and supercoilded double stranded circle
d. found inside the nucleus of the cell
e. covered with ribosomes

38. which of the following staements is FALSE D
a. DNA polymerase jois nucleotides in the 3 ---->5 direction only
b. the leading strand of DNA is made continous;y
c. the lagging strand of DNA is started by and RNA primer
d. DNA replication proceeds in one direction around the bacterial chromosome
e. multiple replication forks are possible on a bacterial chromose

39.. The transfer of DNA from a donor to a recipient cell by a bacteriophage is B
a. conjugartion
b. transduction
c. transformation
d. genetic engineering
e. none of the above

40. which of the follwoing is NOT a product of transcription? A
a. a new strand of DNA
b. rRNA
c.tRNA
d.mRNA
e. none of the above

41. an enzyme produced in response to the presence of a substrate is called A
a. an inducible enzyme
b. a repressible enzyme
c. a restriction enzyme
d. an operator
e. a promoter

42. The DNA probe, GGCTTA, will hybridize with DNA containing – most likely C, but the polarity of all strands should be given in order to answer the question properly
A. CCGUUA
B.CCGAAT
C.GGCTTA
D.GGUUA
E.GGCAAU

43. Which of the following is the reaction catalyzed by DNA - dependent RNA polymerase?
A. DNA----> mRNA
B. mRNA---> cDNA
C.mRNA---> protein
D. DNA ---> DNA
e. none of the above

44. Which of the following enzymes is used in replication of the lagging DNA strand? – Not sure, have to look it up possibly E?
A. RNA polymerase
B. DNA polymerase
C. DNA ligase
D. A and B only
E. A,B and C

45. In transcription B
a. DNA is change to RNA
b. DNA is copied to RNA
c. DNA is replicated
d. RNA is copied to DNA
e. proteins are made

46. the value of cDNA in genetic engineering is that B?
a. it lacks exons
b. it lacks introns
c. it is really RNA
d. it allows prokaryotic genes to be inserted into eukaryotic dna
e. none of the above

USE THE FOLLOWING INFO TO ANSWER QUESTIONS 47-49 - requires a codon table & I’m too lazy to look one up or use one – on your own for those
CONDON ON mRNA and the corresponding amino acid

47. if the sequence of amino acids cpded for by a strand of DNA is valine- histidine proline arginine what is the order of bases in the strand of DNA?
A CAGGTAGACTAC
B. GUUCAUCCGCGU
C.CAAGTAGGCGCA
D.GACGTAACATTT
E.CAGGGCGGCGCA

The antisense ( complimentary) strand coding for the polypeptide in question 47 is
A. GTCCATCTGATG
B.GTTCATCCGCGT
C.GUUCAUCCGCGT
D.GTCCCGCCGCGT
E.GTCCATCTGATG

What os the sequence of mino acids coded for by the following sequence of bases in a strand of DNA? GACTACACATTT
A. Leucine methionine cysteine lysine
B. prline arginine histidine proline
c. leucine lysine arginine valine
d. transcription would stop at the first codon
e. cannont be determined from the data given

What is the anticodon for methionine?
a. TAC
B. GTA
C. AUG
D. CGA
E. UAC

which of the following is not used in make cDNA? D
A. REVERSE TRANCRIPTION
B. RNA processing to remove introns
c. transcription
d, translation
e. i do not know, so I am choosing this wrong answer

Took me all of 20 minutes to do that without a text and I make to claims to know what I'm talking about. It would have taken a little longer if I had bothered to look up a few things and to read things off a codon table. If I'm wrong and you copy down my wrong answer, too bad. See, the point is to learn something, not just to take the bleeping test. - As I see others are trying to say as well.




thanx a bunch ... most of our answers are / were the same except #'s

1,3, 16,31 and 46 !!


YALL ACT LIKE I DIDNT EVEN EFFIN TRY !!!! U ALL CAN SUX MY DICK ... IF I HAD ONE LOL I DID TRY I JUST WANTED TO COMPARE ANSWERS THATS ALL GEEESH ......but overall I think I did pretty well ... and the name of this post isnt "do my homework" ... I simply ASKED for help thats it .... :roll: and thanks again blcr11
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Postby BCH04 » Mon Jun 18, 2007 4:18 pm

Poison wrote:Where, in this site, does it write: "We do your homework for free!!!" Nowhere....
At least TRY to do it yourself.



STFU GOOSH .....


AND I HAVE GRANT U MY PERMISSION TO DELETE THIS POST .... :roll:
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Postby blcr11 » Mon Jun 18, 2007 4:43 pm

Sorry. I don't mean to be too rough on you. It's a problem of sorts when people post what appears to be a homework assignment and don't give too many additional details. You may have had your own answers to the questions--I'll take you at your word--but I would have liked to see what you thought the correct answers were and maybe why. That would have given me a better opportunity to correct (or at least argue about) answers I thought were incorrect. Instead, it's like having a page of 50 questions thrown in my face with a request to give you answers. OK, I know you didn't exactly say it that way, but it feels like it from this end. For myself, I don't have "ethical" problems about giving answers away--for free even--just because a) it's fun and b) it's good review for me. And there's no pressure on me, because I only have to think I know the answer. It would be nice if there was some learning going on, but there's only so much one can do. Either both parties are participants, or they're not. I can only hope that you learned some biology/biochemistry either through this exchange or through your course.
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Postby mith » Mon Jun 18, 2007 7:43 pm

This long post hurts my eyes
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
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Postby david23 » Mon Jun 18, 2007 8:43 pm

BCH stop lying, if u wanetd to compare answers you would have posted the answer in the first place, which would have made things faster if we just pointed out your mistake instead of having to do the whole test. I seriously hope after taking this class for the second time you pass it this time, because biology is not the career for you.
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Postby Poison » Wed Jun 20, 2007 11:06 am

mith wrote:This long post hurts my eyes


yep.....
It matters not how strait the gate
How charged with punishment the scroll
I am the Master of my fate
I am the Captain of my soul.
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