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Wouldn't it be possible to use primers with different tm?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Postby blcr11 » Mon Oct 08, 2007 9:24 pm

Taq should work if the desired product is short-ish. It isn't the least error-prone enzyme around, nor is it the fastest. I prefer KOD Hot Start DNA polymerase from Novagen, but I think Invitrogen has similar high fidelity enzymes available.
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