Join for Free!
121585 members

Wouldn't it be possible to use primers with different tm?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Postby blcr11 » Mon Oct 08, 2007 9:24 pm

Taq should work if the desired product is short-ish. It isn't the least error-prone enzyme around, nor is it the fastest. I prefer KOD Hot Start DNA polymerase from Novagen, but I think Invitrogen has similar high fidelity enzymes available.
Posts: 672
Joined: Fri Mar 30, 2007 4:23 am


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests