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gluconic acid detection by hplc

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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gluconic acid detection by hplc

Postby mslynnlynn » Tue Jun 05, 2007 9:16 am

i want to know which solvent and column type(in HPLC) is used to detect the gluconic acid and 2-ketogluconic acid produced from p solubilizing bateria. i search this from internet.
but i can't find.
please help me.
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Postby microworld » Tue Jun 05, 2007 3:27 pm

hi,i got a paper,hope that can help you
it's a pity that i can post URL'S/LINKS,
i've just pm to you
check it
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thank u

Postby mslynnlynn » Mon Jun 11, 2007 4:31 am

thank u for ur help. but i can't open ur files except abstract. i feel sorry. could u help me to open pdf?
very very thank u.
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Postby kanak33 » Mon Jun 11, 2007 8:48 am

If you want to open .pdf file you need Adobe Reader.
you can download from here:
http://www.adobe.com/products/acrobat/readstep2.html
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Email or IM me!
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Postby mslynnlynn » Mon Jun 18, 2007 4:08 am

amm,
i said i can't open pdf.
but it is not for being absence of adobe.
for the absence of online paying system of our country.thank u for ur advice.
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Postby blcr11 » Mon Jun 18, 2007 5:29 pm

You mean if you actually receive a .pdf file you can't open it because of some non-paying situation in the UK with respect to adobe? Or do you mean that if someone sends you a link to a journal article you can't open anything that requires a subsription to the journal? Send me the citation(s). If I have full access to the journal(s) in question, I can send you a .pdf or .html file of the article(s). If I don't have full access, I can't do any more than open the abstract either, though.
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Postby mslynnlynn » Wed Jun 20, 2007 5:10 am

thank u friend,
i mean i can't open full pdf eventhough someone post abstract.
thank u for ur kind help.
i send two citation related with my question.
Ethyl Methanesulfonate Mutagenesis–Enhanced Mineral Phosphate Solubilization by Groundnut-Associated Serratia marcescens GPS-5
Journal Current Microbiology
Publisher Springer New York
ISSN 0343-8651 (Print) 1432-0991 (Online)
Issue Volume 54, Number 2 / February, 2007
DOI 10.1007/s00284-005-0334-1
Pages 79-84
Subject Collection Biomedical and Life Sciences
SpringerLink Date Wednesday, January 03, 2007
and
Rahnella aquatilis, a bacterium isolated from soybean rhizosphere, can solubilize hydroxyapatite1
Kil Yong KimaaDepartment of Plant Pathology, 108, Waters Hall, University of Missouri, Columbia, MO 65211, USA, Diann JordanbbDepartment of Soil and Atmospheric Science, University of Missouri, Columbia, MO 65211, USA, Hari B. Krishnana,aDepartment of Plant Pathology, 108, Waters Hall, University of Missouri, Columbia, MO 65211, USA**Corresponding author. Tel.: +1 (573) 882-2862; Fax: +1 (573) 882-0588; E-mail: Krishnan@PSU.Missouri.eduaDepartment of Plant Pathology, 108, Waters Hall, University of Missouri, Columbia, MO 65211, USAbDepartment of Soil and Atmospheric Science, University of Missouri, Columbia, MO 65211, USA
*Corresponding author. Tel.: +1 (573) 882-2862; Fax: +1 (573) 882-0588; E-mail: Krishnan@PSU.Missouri.edu
1Contribution from the Missouri Agricultural Experiment Station Journal No. 12,627.

Abstract

A diazotropic bacterium, ISL19, has been isolated from soybean rhizosphere. ISL19 grows well at 30°C and utilizes diverse carbon sources for its growth. This Gram-negative bacterium is rod-shaped, is about 2–3 μm in size and contains several flagella. On the basis of its cellular fatty acid profile, its carbon utilization pattern, and the nucleotide sequence of a conserved segment of a 16S rRNA gene, ISL19 has been identified as Rahnella aquatilis. This bacterium exhibited a strong ability to solubilize hydroxyapatite and dicalcium phosphate in the external culture medium. The solubilization of hydroxyapatite was associated with a drop in the pH of the culture medium. The change in the pH value showed an inverse correlation with the soluble phosphorus concentration. Analysis of the culture medium by high pressure liquid chromatography identified gluconic acid as the main organic acid released by R. aquatilis.

This article is cited by:
Z. Rengel and P. Marschner. (2005) Nutrient availability and management in the rhizosphere: exploiting genotypic differences. New Phytologist 168:2, 305–312
Abstract Abstract and References Full Text Article Full Article
thank u.
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Postby blcr11 » Wed Jun 20, 2007 2:52 pm

I only have full access to the second paper. I've sent you a pdf file of the full paper (not just the abstract). If you cannot open the full paper for some reason I can either send the html version (though I kind of think the figures won't be there when you open it) or I can copy the body of the text directly into an e-mail--at least I think I can. You can also contact the authors and request a reprint--the good old fashioned way of doing this kind of thing.
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