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microscopic tools

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microscopic tools

Postby mehdi71000 » Sun Jun 03, 2007 1:51 pm

Hi I have a problem isolating plant cells. Are three microscopic tools for this process or chemicals is used?
Thanks :D

One more thing in micro injection there is a device witch holds the cell. What is this device called? Thanks
Last edited by mehdi71000 on Sun Jun 03, 2007 10:04 pm, edited 2 times in total.
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Postby canalon » Sun Jun 03, 2007 8:06 pm

What you ae looking for is amicromanipulator. If I were you I would start winning lotteer right now...
Patrick

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Postby david23 » Sun Jun 03, 2007 8:18 pm

this is one of the equipments on your list that u cant afford. For what you are doing, just get a microscope and observe a seed, or a plant that is starting to sprout. and use your microinjector on the cells you can see.
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Postby mehdi71000 » Sun Jun 03, 2007 8:47 pm

No I’ve got every thing ready
Ok here is the list of what I bought

900X computer microscope
2 micro pumps one for injections and other for suction to hold the cell
20 needles very fine 0.5 um outer diameters
2 micropositioner. For guiding the needle
Cell culture kit (agar nutrient and hormones and stuff)
I think all I need is a needle to hold a cell with and the Luc vector. And a pregnant fish I’m hoping I can do the experiment with snails since they breed like mad in my garden. I’m also what to do this experiment on bacteria and small plants
All this equipment cost me something about
£300 from eBay
Last edited by mehdi71000 on Sun Jun 03, 2007 9:57 pm, edited 1 time in total.
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Postby david23 » Sun Jun 03, 2007 9:55 pm

lets try it with snails then.
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Postby mehdi71000 » Sun Jun 03, 2007 10:03 pm

Any ideas on how to get then to mate. I watch them yesterday for 1 hour they were ready to mate but no dart to inject the sperm. Maybe I can open one and extract the sperm go from there.
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Postby arian » Mon Jun 04, 2007 6:26 am

you may use some of lust solution on them.
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Postby david23 » Mon Jun 04, 2007 8:43 am

you dont have the equipment for fertilizing on your own.
Just capture an infant snail, and do it on it. The result wont be as good as the embryo one.
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Postby mehdi71000 » Mon Jun 04, 2007 8:56 am

ok, thanks guys for your replies
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