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replacing Cells in and outs

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replacing Cells in and outs

Postby mehdi71000 » Fri Jun 01, 2007 12:50 am

Hi everyone
Any good sources of luciferin expression vector?
and
I have this crazy idea. Is it possible to remove all the dna from a cell and insert another species dna and remove all cell parts and add new cell parts. Would the cell work? You might ask why. I thought it might be interesting. If so could I do the same but inserting a bacteria in a plant cell. Thought this might not be possible since cell walls have protein in them witch transfer food to the cell and plant cells might need different nutrients.
Thanks for your help
Last edited by mehdi71000 on Tue Jun 05, 2007 1:43 pm, edited 1 time in total.
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Postby schizophrenicsinner » Fri Jun 01, 2007 5:05 pm

Don't become all Dr. Jekell & Mr. Hyde !

DNA is not to be tampered with: I don't know what your teachers tell you, but don't!

A doctor's vow is to heal: For this you fix & let heal... Don't tamper with nature.

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Postby MrMistery » Fri Jun 01, 2007 7:49 pm

cloning is something like this: take out the nucleus from an egg cell and put in a diploid one from a somatic cell. However, it must be from a cell of the same species. Putting a bacteria in a plant cell will lead to immediate cell death, no doubt about that.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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Postby mehdi71000 » Fri Jun 01, 2007 11:51 pm

thanks guys
is there an expeiment i can do in microinjection that does not require vector injection. i need this becouse the vector i orderd is going to take few weeks to arive.
thanks
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Re: replacing Cells in and outs

Postby satrohraj » Sat Jun 02, 2007 2:28 am

mehdi71000 wrote:Hi everyone
I have this crazy idea. Is it possible to remove all the dna from a cell and insert another species dna and remove all cell parts and add new cell parts. Would the cell work? You might ask why. I thought it might be interesting. If so could I do the same but inserting a bacteria in a plant cell. Thought this might not be possible since cell walls have protein in them witch transfer food to the cell and plant cells might need different nutrients.
Thanks for your help


It will surely lead to death if the DNA is not compatible, which is the most possible case!
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Postby mehdi71000 » Sat Jun 02, 2007 10:27 am

What if a cell dna and other parts are removed and your only left with the nucleus wall and the cell wall. Why would the cell die since its all molecules? Does the cell not function at all? Is that called a dead cell? What if after cells death some one injected the same spices dna and cell materials. Would the cell function again?
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Postby blcr11 » Sat Jun 02, 2007 12:58 pm

There were some classic experiments done--I forget exactly when, possibly as early as the early 1900s--involving the transfer of the nucleus of one cell type into a de-nucleated cytoplasm of another cell either of the same or different type. My (now dim) recollection of the experiments is that they were trying to see which was dominant in development, the nucleus or the cytoplasm. They may also have looked at or for cytoplasmic factors affecting the cell cycle--can't say for sure anymore. The point is that similar experiments have been done--though not between plant and animal cells that I recall. My guess is you could get it to work maybe between different types of animal cells, though how distant a relationship could be tolerated I couldn't say. I'm skeptical it would work between plant and animal cells. It's also not immediately clear to me what you could do with such hybrid cells, but I'm willing to be convinced. Re using "dead" cells--there I doubt anything would work. Well, how dead? Nearly dead or mosty dead? If you transfer a functional nucleus into a non-functional cytoplasm, how can there be any translation? And conversely, if you put a dead nucleus into a fully functional cytoplasm, where will the mRNA come from to instruct the protein synthetic machinery?
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Postby mehdi71000 » Sat Jun 02, 2007 3:23 pm

I thought cells and protein work as a result of chemical reactions. So I assumed a dead cell is a cell that its chemical reactions are distorted some how and it cant function. if this is true if I remove the nucleus content and the outer nucleus content the walls will still be functional but with out a energy source would be dysfunctional.
I all so though if a cell dna is changed if it duplicates would the new wall be the wall of the introduced dna. I mean the dna is responsible for the building of the cell wall so with the new materials how can the cell wall split to form 2 cells? I think I didn’t thought to much about this topic before I asked a bout it. I assume every living thing cell walls function differently when the cell splits. is this true?
Any cheap sources of lusfres vector those that glow ?

Thanks for your replies
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Postby david23 » Sat Jun 02, 2007 4:49 pm

how do I answer this one
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Postby blcr11 » Sat Jun 02, 2007 5:59 pm

An organism dies and, for some period of time, individual cells may remain functional. That’s why it is possible to transplant an organ from a “fresh” cadaver. When an individual cell dies—whether it dies “naturally” or due to external damage—it has lost essential functions: its chromosomes have lost the ability to pair or divide, or the membranes are compromised such that metabolism is not possible, or—who knows exactly why the cell is no longer viable. Some enzymes or structural proteins may remain more or less intact (but probably not for long since one of the things that happens pretty quickly is lysosomal disintegration and release of lytic enzymes into the surroundings accelerating the loss of functional proteins) but the logic is missing. It’s hard for me to imagine restoring enough function by the experiment you’re proposing. I wouldn’t categorically deny the possibility, but I think you’d have a hard time convincing NIH or NSF to fund you.

Invitrogen, Clontech, Promega, possibly Roche Diagostics sell luciferase related things—but I doubt any of them will be all that cheap.
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Postby mehdi71000 » Sat Jun 02, 2007 9:20 pm

Thanks man you’re a life saver
in these two sites witch is best for making glowing plants and glowing animals by luciferase vector?

Site A:

http://www.promega.com/search/Default.a ... +vector%22

Site B:


http://www.promega.com/catalog/catalogp ... cer+Vector

site C:

http://www.promega.com/catalog/catalogp ... say+System


i think site B is suitable. But I’m not sure. I’ve read and they said I think its for mammalian animals :? Could I use it on plants in cell culture and bacterias aswell
Thanks guys


pGL2-Enhancer Vector

http://www.promega.com/tbs/tm003/tm003.html
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Postby blcr11 » Sun Jun 03, 2007 2:53 am

Try this: http://www.promega.com/pnotes/44/luerhsen/luerhsen.html

My guess is you can't use a mammalian cell-specific promoter in a plant cell, but what do I know about plants?
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